abstract during a survey for detection of pvs in potato fields in northern khorasan province, some tubers from the plants which have been already marked and showing mosaic, rugosity, undulation on leaf margins, little leaf and slightly vein deepening, were collected in bodjunrd region, also, some tubers from potato fields of mashhad, chenaran, ghoochan, faruj and shirvan regions were randomly c...

The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific f...

beet necrotic yellow vein virus (bnyvv) is the causal agent of sugar beet rhizomania, disease .  in the present study which was conducted in 2005 in shiraz, iran, serological methods (including elisa) were used for detection of disease and screening of disease tolerant germplasm in sugar beet breeding programs. for doing this, iranian isolate of bnyvv was purified and a specific antiserum was p...

Antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the presence seed transmissibility of bean common mosaic virus-blackeye cowpea (BCMV-BICM) in farm- retained lots obtained from 46 locations, including markets farms major growing areas Ashanti Brong Ahafo regions Ghana. In growout tests, virus symp...

(Di)bromotyrosine is formed by the specific reaction of eosinophil peroxidase and can be used as an eosinophil activation marker. In the present study, an antibody for (di)bromotyrosine in proteins was prepared to investigate the pathogenesis of eosinophil-related diseases such as allergic responses. A rabbit polyclonal antibody was raised against brominated keyhole limpet hemocyanin. The speci...

Infeksi Papaya ringspot virus (PRSV) di Indonesia pertama kali dilaporkan pada pepaya Nangroe Aceh Darussalam tahun 2012. Sejak itu, PRSV atau penyakit bercak bercincin menyebar ke beberapa daerah Jawa, Sumatera, Bali, dan Nusa Tenggara Barat. Penelitian ini dilakukan untuk mengonfirmasi keberadaan penanaman Jawa mengetahui identitas molekulernya. Metode double antibody sandwich enzyme-linked i...

Pelargonium zonate spot virus (PZSV) is identified recently in tomato plants in the United States. To develop serological diagnostic tools for the detection of this virus, the production of good quality antibodies is a necessity. The coat protein (CP) gene of a California isolate of PZSV was cloned into a bacterial expression vector (pTriEX-4 Ek/LIC). The plasmid pTriEX-4-PZSV-CP was transforme...

Since the introduction of our plasma cortisol enzyme-linked immunosorbent assay (ELISA) [l] using affinity purified antiserum [2] into the routine steroid laboratory, we have embarked on the development of a simple urinary free or unconjugated cortisol ELISA which would allow measurement of both urine and plasma cortisol on the one ELISA plate. This paper describes for the first time an ELISA f...