materials and methods: in order to achieve this, recombinant plasmid harboring antisense sequences targeting both of pta and acka was assembled, after transfecting to the cells, its effects on the cell growth and acetate accumulation in the minimal media was assessed and compared with the control, the plasmid without antisense cassette, in presence and absence of iptg in escherichia coli bl21 (...

The insulin-like growth factor I receptor (IGF-IR) plays an essential role in the establishment and maintenance of transformed phenotype. Interference with the IGF-IR pathway by antisense causes reversal of the transformed phenotype in many rodent and human tumor cell lines. We stably transfected the PC-3 human prostate cancer cell line with an IGF-IR antisense RNA expression plasmid. The ...

the insulin-like growth factor i receptor (igf-ir) plays an essential role in the establishment and maintenance of transformed phenotype. interference with the igf-ir pathway by antisense causes reversal of the transformed phenotype in many rodent and human tumor cell lines. we stably transfected the pc-3 human prostate cancer cell line with an igf-ir antisense rna expression plasmid. the numbe...

Antisense oligonucleotides (ASOs) are a promising clinical tool that could be applied for unmet medical needs, but there several limitations their therapeutic application. Here, we designed and synthesized ( S )-5?- C -aminopropyl-2?- O -methylcytidine, containing -methyluridine -methylcytidine. We then investigated the properties of ASOs these nucleoside analogs. -Aminopropyl modifications enh...

The recent identification of antisense RNA in the transcriptomes of many eukaryotes has generated enormous interest. The presence of antisense RNA in Plasmodium falciparum, the causative agent of severe malaria, remains controversial. Elucidation of the mechanism of antisense RNA in P. falciparum synthesis is critical in order to demonstrate the origin and function of these transcripts. Therefo...

Long noncoding RNAs (lncRNAs) are increasingly recognized as involved in human physiology and disease. Antisense lncRNAs ubiquitous the genome have many mechanisms of action proposed, but single-cell single-molecule information that is needed to support or refute these lacking. Although single molecule RNA-Fluorescent Situ Hybridization (RNA-FISH) a method sometimes used investigate lncRNAs, co...

Abstract Using RNA antisense purification and mass spectrometry, we identified more than 100 human proteins that directly specifically bind SARS-CoV-2 in infected cells. To gain insights into the functions of selected interactors, applied genetic perturbation pharmacological inhibition experiments, mapped contact sites on viral RNA. This led to identification host dependency factors defense str...

Expression of foreign proteins in E. coli is normally inhibited by exogenous production of acetate. To overcomethis problem, various strategies have been proposed and tested to reduce the extent of acetate accumulation.Although these strategies can improve the outcome, the implementation of their proposed techniquesis not practical. Because to achieve optimal results, it requi...