h.pylori infection stimulates immune responses. these responses at the mucosal level are predominantly of iga types, while circulating antibodies against this microorganism are predominantly igg classes. igm antibodies are rarely found and seem to be non-specific for this bacterium. in this research, water extract antigen, from three strains of h.pylori (isolated from patients with gastritis, d...

Background: Celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. However, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. The seronegative patients with celiac disease can i...

background: cystic echinococcosis (ce) is one of the most predominant parasitic zoonosis infections in the world. due to the high recurrence rate of the disease after surgery, follow up of the patient is necessary. the aim of current research was to evaluate the sensitivity of elisa method for hydatid antibodies detection in confirmed ce cases before and its pattern after surgery. methods: the ...

background: celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. however, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. the seronegative patients with celiac disease can i...

The aim of the present was to evaluate the prevalence of H9N2-specific antibodies among water buffaloes (Bubalus bubalis). To this end, blood samples were obtained from 80 randomly selected water buffaloes, 40 cases of which were obtained in the winter months, and 40 cases were sampled in the spring months. The presence of H9N2-specific antibody was determined by hemaggl...

The aim of this study was to investigate the hemagglutination properties of caprine herpesvirus-1(CpHV-1) and their application for CpHV-1 antigen detection and evaluation of the dynamics of postinfectious/vaccine antibody production. CpHV-1 hemagglutination activity was proved only with mouseerythrocytes, PBS and saline solutions at 4°C and 22°C. In vaccinated animals, goats and bucks withresp...

Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...

Antibody  based  methods  have  numerous  advantages  compared  to  other  detection  methods. However, heavy metals cannot stimulate the immune antibody response; it is the main obstacle for preparation of the antibodies that used in detection methods of the metals. The production of the immunogenic Pb complexes by using cost benefit linkers of EDTA and DTPA were investigated in this  study.BS...

objective : the aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (dlmaa), using non-radioactive organic synthetic dyes for identification of toxic shock syndrome toxin-1 (tsst-1) producing strains of staphylococcus aureus. materials and methods: the assay protocol required only two simple steps; addition of tsst-1 antigen to a nitrocellulose membrane and then ad...