Globally, anthrax outbreaks pose a serious threat to people, livestock, and wildlife. Furthermore, environmental change can exacerbate these outbreak dynamics by altering the host–pathogen relationship. However, little is known about how quantitative spatial of host movement may affect spread Bacillus anthracis, causative agent anthrax. Here, we use real-time observations high-resolution tracki...

Distressing effects on animal and human health with lethal progression, being used as bioweapon shared features non-pathogenic bacteria demands sensitive, specific, safe, cost effective rapid detection methods for anthrax causing organisms. Conventional microbiology based diagnostics are time consuming need sophisticated equipment, while molecular require less labor. The Loop mediated isotherma...

abstract snp typing is now a well-established genotyping system in bacillus anthracis studies. in the original standard method of van erth, snps at 13 loci of the b. anthracis genome were analyzed. in order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding snps were designed. besides, a universal pcr protocol...

background and objectives: bacillus anthracis is one of the most homogenous bacteria ever described. bacillus anthracis 17jb is a laboratory strain. it is broadly used as a challenge strain in guinea pigs for potency test of anthrax vaccine. material and methods: this work describes genetic characterization of b. anthracis 17 jb strain using the snps and mlva genotyping. results and conclusion:...

Keywords: Bacillus anthracis, diagnostics, identification, rapid tests, microbiological studies, methods of molecular microbiology

Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has recently become difficult to identify B. anthracis by using previously reported molecular-based meth...

The ability of the endospore-forming, gram-positive bacterium Bacillus anthracis to survive in activated macrophages is key to its germination and survival. In a previous publication, we discovered that exposure of primary murine macrophages to B. anthracis endospores upregulated NOS 2 concomitant with an .NO-dependent bactericidal response. Since NOS 2 also generates O(2).(-), experiments were...

A fluorescein-conjugated antibody against formalin-inactivated spores of Bacillus anthracis Vollum reacted only weakly with a variety of Bacillus species in microfluorometric immunofluorescence assays. A conjugated antibody against spores of B. anthracis Sterne showed little affinity for spores of several B. anthracis isolates including B. anthracis Vollum, indicating that more than one anthrax...

Bacillus anthracis, the causative agent of anthrax, is known for its rapid proliferation and dissemination in mammalian hosts. In contrast, little information exists regarding the lifestyle of this important pathogen outside of the host. Considering that Bacillus species, including close relatives of B. anthracis, are saprophytic soil organisms, we investigated the capacity of B. anthracis spor...