This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide solution followed by flow cytometry analysis. It is based on the principle that normal cells are hydrophobic in nature as they express phosphatidyl serine in the inner membrane (side facing the cytoplasm) and when the cells...

leukemia is a blood disease which is caused by the inhibition of differentiation and an increased proliferation rate. nature has been known as a rich source of medically useful substances. high diversity of bioactive molecules extracted from marine invertebrates, makes them ideal candidates for cancer researches. the study has been done to investigate cytotoxic effects of dichloromethane brittl...

Leukemia is a blood disease which is caused by the inhibition of differentiation and an increased proliferation rate. Nature has been known as a rich source of medically useful substances. High diversity of bioactive molecules extracted from marine invertebrates, makes them ideal candidates for cancer researches. The study has been done to investigate cytotoxic effects of dichloromethane brittl...

guish between apoptotic and necrotic cells, we performed an Annexin V assay with propidium iodide exclusion. In accordance with our TUNEL assay, .95% of the cells were Annexin V positive (unpublished results). Less than 5% of these Annexin V-positive cells showed simultaneous incorporation of propidium iodide. We therefore conclude that .95% of the plasmaderived cells are indeed in apoptosis an...

the cytotoxic activity of natural killer cells is usually tested by radioactive assay csi cr release assay), which detects the release of cytoplasmic contents after plasma membrane disintegration of dying cells. in contrast to this indirect evaluation of cytotoxicity, the assessment of cell damage by flow cytometry aims to provide a more exact characterization of the death pathway via detection...

Melanoma has been reported a contemporary malignant cancer. Here, anti-cancer effects of sea cucumber extract (SCE) have been examined on melanoma cells and compared with dacarbazine. MTT assay and morphological analysis were performed to evaluate cytotoxic effects of methanolic SCE Also, several methods were exerted to detect cell dying by SCE and dacarbazine. The MTT assay showed that B16F10 ...

Leukemia is a blood disease which is caused by the inhibition of differentiation and an increased proliferation rate. Nature has been known as a rich source of medically useful substances. High diversity of bioactive molecules extracted from marine invertebrates, makes them ideal candidates for cancer researches. The study has been done to investigate cytotoxic effects of dichloromethane brittl...

The cytotoxic activity of natural killer cells is usually tested by radioactive assay CSI Cr release assay), which detects the release of cytoplasmic contents after plasma membrane disintegration of dying cells. In contrast to this indirect evaluation of cytotoxicity, the assessment of cell damage by flow cytometry aims to provide a more exact characterization of the death pathway via detec...

Zerumbone (ZER), a natural compound has been extracted from Zingiber zerumbet with known pharmacological activities. The aim was to determine the anti-human Burkitt’s lymphoma (Raji) cell effect of ZER. 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay used cytotoxic while Annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays apoptotic ZER on hu...