conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...

Imprecise usage of the terms "sensitivity" and "specificity" produces confusion in the diagnostic use of sophisticated laboratory test results. "Analytical sensitivity" represents the smallest amount of substance in a sample that can accurately be measured by an assay. "Analytical specificity" refers to the ability of an assay to measure on particular organism or substance, rather than others, ...

developed in 1991, nucleic acid sequence-based amplification (nasba) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than pcr, and it can also be more sensitive. this paper describes the development of a molecular beacon-based multiplex nasba assay for simultaneous detection of hiv-1 and hcv in plasma samples.a well-conserved region...

We evaluated analytical factors such as antibody specificity, standard materials, and methodology for the measurement of C3. Mancini-type radial immunodiffusion and immunonephelometry were shown to give comparable data if variables other than procedural variables are eliminated. The most significant analytical factors affecting the measurement were antiserum specificity and source of standard m...

OBJECTIVE Our objective was to verify the analytical performance of the Afirma gene expression classifier (GEC) in the classification of cytologically indeterminate thyroid nodule fine-needle aspirates (FNAs). DESIGN Analytical performance studies were designed to characterize the stability of RNA in FNAs during collection and shipment, analytical sensitivity as applied to input RNA concentra...

I propose a method for defining tolerance limits for assay bias and assay imprecision, based on the effects of these tolerance limits on the clinical specificity of the assay. An analytical "error budget" is defined as the squared sums of the imprecision and bias errors. The maximum limit for this error budget is set at a value corresponding to a 50% increase in the false-positive rate for clas...

Drink-drivers frequently allege that their breath alcohol reading was so high because some non-ethanolic chemical was in their breath at the time of the test. This paper reviews the author’s experiences and methodology in dealing with such matters in the British Courts on behalf of the Police. Conceivable sources of breath contaminants are ingestion (with food, medication or smoking), endogenou...

Background: Chest radiography is one of the most usual diagnostic tools for respiratory distress. Objective: The purpose of this study is to assess the specificity, sensitivity and clinical value of chest radiography of neonates with respiratory distress.Patients and Methods: A descriptive- analytical study was conducted on 102 neonates that were in neonatal intensive care unit of Imam Reza and...

We studied the specificity of kinetic methods of analysis, with emphasis on creatinine determination. The error contributed by an interferent that reacts with the analytical reagent and absorbs at the wavelength of analysis is shown to be related to the ratio of the equilibrium absorbances of the analyte and interferent, the ratio of the rate constants, the extent of the analytical reaction, an...

background: chest radiography is one of the most usual diagnostic tools for respiratory distress. objective: the purpose of this study is to assess the specificity, sensitivity and clinical value of chest radiography of neonates with respiratory distress.patients and methods: a descriptive- analytical study was conducted on 102 neonates that were in neonatal intensive care unit of imam reza and...