acanthamoeba keratitis is a vision-threatening infection caused by pathogenic species of the genus acanthamoeba. in this study, 13 acanthamoeba keratitis cases were diagnosed among 52 keratitis patients. to confirm the identity of acanthamoeba at the genus level, a pcr-based method was used, and their pathogenic potential was determined using in vitro cytotoxicity assays on human corneal epithe...

background: poor hygiene will provide good condition for corneal infections by opportunistic free-living amoebae (fla) in soft contact lens wearers. in the present study an amoebic keratitis due to hartmannella has been recognized in a 22-year-old girl with a history of improper soft contact lens use. she had unilateral keratitis on her left eye. her clinical signs were eye pain, redness, blurr...

background: amoebic keratitis introduced as a painful corneal infection which sometimes lead to poor vision and blind-ness. the main goal of this study was to report amoebic keratitis during ten years from 1997-2007 in patients who was sus-pected to have amoebic keratitis and referred to parasitology laboratory, school of public health, tehran univer¬sity of medical sciences, iran. other aim wa...

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene b...

In order to evaluate the possible roles of secretory proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by Acanthamoeba lugdunensis KA/E2, isolated from a Korean keratitis patient. The ammonium sulfate-precipitated culture supernatant of the isolate was purified by sequential chromatography on CM-Sepharose, Sephacryl S-200, and mono Q-an...

Keratitis is an inflammatory condition, characterized by involvement of corneal tissues. Most recurrent challenge of keratitis is infection. Bacteria, virus, fungus and parasitic organism have potential to cause infection. TLR are an important class of protein which has a major role in innate immune response to combat with pathogens. In last past years, extensive research efforts have provided ...

A 22-year-old farmer with recurrent corneal erosions and keratoiritis underwent a penetrating keratoplasty. Investigations carried out on the corneal disc revealed an amoebic keratitis, which is considered to be a very rare condition.

Naegleria fowleri, Acanthamoeba spp., Balamuthia mandrillaris, and Sappinia sp. are pathogenic free-living amoebae. N. fowleri causes Primary Amoebic Meningoencephalitis, a rapidly fatal disease of the central nervous system, while Acanthamoeba spp. and B. mandrillaris cause chronic granulomatous encephalitis. Acanthamoeba spp. also can cause cutaneous lesions and Amoebic Keratitis, a sight-thr...

Acanthamoeba species are free-living amoebae that can be found in almost every range of environments. Within this genus, a number of species are recognized as human pathogens, potentially causing Acanthamoeba keratitis, granulomatous amoebic encephalitis, and chronic granulomatous lesions. Soil and water samples were taken from experimental station at Julianna Major of Plant Protection Institut...