parasitic colitis, especially entamoeba histolytica, is a well-known disorder in the middle east particularly in iran. the carrier state of the disease or the chronic type frequently remains undiagnosed or mistaken as irritable bowel syndrome which results in further discomfort and increases the incidence of associated complain. we devised a technique by inserting bisacodyl suppository and then...

AIMS To develop a rapid latex agglutination screening test for invasive amoebiasis. METHODS The performance of an in-house latex agglutination test was compared with three standard serological techniques--the immunofluorescent antibody test (IFAT), the indirect haemagglutination test (IHA), and the cellulose acetate precipitin (CAP) test. Forty six sera were screened; 12 from negative control...

The demonstration of antibodies in syphilitic sera that will immobilize Treponema pallidum (Nelson and Mayer, 1949) led Cole and Kent (1953) to investigate and demonstrate that immune rabbit sera were capable of immobilizing Entamoeba histolytica, and to suggest that sera from human cases of amoebiasis might show the same phenomenon. Their rabbit sera did not show immobilizing activity to a hig...

Peripheral blood polymorphonuclear leucocytes (PMN) from patients with invasive amoebiasis, i.e. amoebic liver abscess (ALA) and acute amoebic dysentery, showed marked elevation of nitroblue tetrazolium dye (NBT) reduction. This dramatic change was not observed in PMN from patients with non-invasive amoebiasis, i.e. non-suppurative hepatic amoebiasis, or in asymptomatic Entamoeba histolytica cy...

A positive serological diagnosis of amoebiasis could be made by immunofluorescence in 66 of 78 established cases, taking a serum titre of 16 or higher as diagnostic: at this level there were no false positives among 94 control sera. The test is simple and may be carried out on amoebic smears stored for several months in 2-octanol. The serological activity is largely confined to the IgG immunogl...

SUMMARY The results of direct stool examination in 102 watery and bloody diarrhea patients were compared with the presence of antibodies against Entamoeba histolytica with Indirect Fluorescent Antibody Test. Besides 22 healthy individuals included in this study as control group. It was found that 22 patients (21.5%) with a sera titre higher than 1/320 bad only 9 (8.8%) positive E. Histolytica ...

We compared the capability of rapid enzyme immunoassay (EIA) to detect antiamoebic antibodies during hepatic amoebiasis with those of indirect hemagglutination and latex agglutination. EIA is simple to perform and rapid (20 min) and does not require any special equipment (optical reading is sufficient). EIA of 143 sera (including 43 from patients with proven hepatic amoebic abscess, 33 from pat...