A completely randomized experimental design was conducted to estimate ammonia excretion rate by red tilapia (Oreochromis sp.) during different stages of its growth in recirculating water system units typically designed to meet the experiment purposes. Eight weight groups each containing 75individuals of 20, 30, 40, 60, 80, 120, 180 and 200g average weight were considered as treatments in duplic...

A completely randomized experimental design was conducted to estimate ammonia excretion rate by red tilapia (Oreochromis sp.) during different stages of its growth in recirculating water system units typically designed to meet the experiment purposes. Eight weight groups each containing 75individuals of 20, 30, 40, 60, 80, 120, 180 and 200g average weight were considered as treatments in duplic...

Dietary protein restriction has multiple benefits in kidney disease. Because protein intake is a major determinant of endogenous acid production, it is important that net acid excretion change in parallel during protein restriction. Ammonia is the primary component of net acid excretion, and inappropriate ammonia excretion can lead to negative nitrogen balance. Accordingly, we examined ammonia ...

In their native environment, gulf toadfish excrete equal quantities of ammonia and urea. However, upon exposure to stressful conditions in the laboratory (i.e. crowding, confinement or air exposure), toadfish decrease branchial ammonia excretion and become ureotelic. The objective of this study was to determine the influences of cortisol and ammonia on ammonia excretion relative to expression o...

Rhesus (Rh) glycoproteins are ammonia gas (NH(3)) channels known to be involved in ammonia transport in animals. Because of the different osmoregulatory and ionoregulatory challenges faced by teleost fishes in marine and freshwater (FW) environments, we hypothesized that ammonia excretion strategies would differ between environments. Also, we hypothesized that cutaneous NH(3) volatilization in ...

Remarkably little is known about nitrogenous excretion in freshwater invertebrates. In the current study, the nitrogen excretion mechanism in the carnivorous ribbon leech, Nephelopsis obscura, was investigated. Excretion experiments showed that the ribbon leech is ammonotelic, excreting 166.0 ± 8.6 nmol·grams fresh weight (gFW)(-1)·h(-1) ammonia and 14.7 ± 1.9 nmol·gFW(-1)·h(-1) urea. Exposure ...

THE FIELD OF TRANSPORT PHYSIOLOGY was fundamentally changed by the discovery of transport proteins that are specific for small neutral molecules such as urea, water, ammonia, and CO2. The identification of first urea transporters (12), then aquaporins or water channels (1), and most recently ammonia transporters (8) (that may also facilitate the movement of CO2), challenged the long-held view t...

The Rhesus factor protein, Rh C glycoprotein (Rhcg), is an ammonia transporter whose expression in the collecting duct is necessary for normal ammonia excretion both in basal conditions and in response to metabolic acidosis. Hypokalemia is a common clinical condition associated with increased renal ammonia excretion. In contrast to basal conditions and metabolic acidosis, increased ammonia excr...

Glutamine synthetase (GS) catalyzes the recycling of NH4 (+) with glutamate to form glutamine. GS is highly expressed in the renal proximal tubule (PT), suggesting ammonia recycling via GS could decrease net ammoniagenesis and thereby limit ammonia available for net acid excretion. The purpose of the present study was to determine the role of PT GS in ammonia metabolism under basal conditions a...

BACKGROUND AND AIMS In patients with cirrhosis, hepatic encephalopathy is often precipitated by dehydration. This study tests the hypothesis that volume expansion in cirrhotic patients increases renal ammonia excretion. PATIENTS AND METHODS Sixteen well compensated cirrhotic patients (mean Pugh score 6.7 (SEM 0.4)) were studied after an overnight fast. One litre of 0.9% saline was administere...