The amanitins (namely ?- and ?-amanitin) contained in certain mushrooms are bicyclic octapeptides that, when ingested, responsible for potentially lethal hepatotoxicity. M101 is an extracellular hemoglobin extracted from the marine worm Arenicola marina . It has intrinsic Cu/Zn-SOD-like activity currently used as oxygen carrier organ preservation solutions. Our present results suggest that migh...

A number of mutant Chinese hamster ovary (CHO) cell lines resistant to the cytotoxic action of alpha-amanitin have been isolated. The alpha-amanitin sensitivity of the different mutant cell lines varied widely, but correlated well with the alpha-amanitin sensitivity of the RNA polymerase II activity in each of these mutant cell lines. In comparison with the RNA polymerase II of wild-type cells,...

A number of mutant Chinese hamster ovary (CHO) cell lines resistant to the cytotoxic action of n-amanitin have been isolated. The a-amanitin sensitivity of the different mutant cell lines varied widely, but correlated well with the au-amanitin sensitivity of the RNA polymerase II activity in each of these mutant cell lines. In comparison with the RNA polymerase II of wild-type cells, three muta...

In herpes simplex virus type 1 (HSV-1)-infected HEp-2 cells, amanitin added before or at various times after infection always reduced viral multiplication. Also, the three waves of transcription of HSV-1 DNA, which led to the synthesis of alpha, beta-, and gamma-polypeptides, were all sensitive to amanitin in HEp-2 cells, and the amanitin-sensitive RNA polymerase activities of isolated nuclei w...

Trastuzumab conjugated to ?-amanitin kills HER2-low breast cancer cells with loss of chromosome 17p and enhances checkpoint blockade therapy.

Low concentrations (0.10 to 1.0 microgram/ml) of alpha-amanitin inhibit wheat embryo germination. Labeled RNA, synthesized in vivo by embryos imbibed in the presence of [3H]uridine and various concentrations of alpha-amanitin, was analyzed by oligo(dT)-cellulose chromatograhy and by gel electrophoresis (acrylamide and agarose) coupled with fluorography. Low concentrations of alpha-amanitin (0.1...

A 10-year-old, female spayed Labrador Retriever was referred for acute hepatopathy and urinary retention. Blood work from the initial presentation (day 0) revealed a severe, mixed hepatopathy. Over course of patient’s hospitalization, patient developed liver insufficiency. Urine submitted toxicological screening detection trace concentration alpha-amanitin. The treated supportively alpha-amanit...

The alpha-, beta-, and gamma-amanitin content of 11 samples of Amanita verna Bull., collected during 1975--1978 in Germany and Switzerland, has been determined by high performance thin-layer chromatography (HPTLC) of crude methanolic extracts. The toxicity (i. v. LD50 for mice of defatted, lyophilized, methanolic extracts) of 3 samples has been compared with that of A. phalloides from the same ...

Amanitin poisonings are among the most life-threatening mushroom poisonings, and mainly caused by genus Amanita. Hepatotoxicity is hallmark of amanitins, powerful toxins contained in these mushrooms, can require liver transplant. Among amatoxins, ?-amanitin studied. However, hypothesis a possible metabolism amanitins still controversial this pathophysiology. Therefore, there need clarification ...

A specific inhibitor of RNA polymerase II, α-amanitin is broadly used to block transcriptional activities in cells. Previous studies showed that α-amanitin affects in vitro maturation of cumulus-oocyte-complex (COC). In this study, we evaluated the target of α-amanitin, and whether it affects oocytes or cumulus cells (CCs), or both. We treated α-amanitin with different time period during in vit...