The E46(+) strain of Adenovirus 7 is a mixed-virus population containing defective Adenoviurs 7-SV40 hybrid particles and helper, nonhybrid Adenovirus 7 particles. We have applied electron microscopic mapping techniques to obtain a physical map of the genome of the hybrid particles present in E46(+)PL1, a substrain of E46(+) derived from a single two-hit plaque. DNA molecules extracted from pur...

The adenovirus 7-simian virus 40 hybrid virus population E46(+) was subjected to fixed-angle equilibrium density gradient centrifugation in CsCl. A difference in buoyant density between the hybrid virion and its nonhybrid adenovirus 7 counterpart was noted, the hybrid virion possessing the lower buoyant density. This difference in buoyant density appeared to be accentuated in a population of ad...

The existence of virus particles in a strain of adenovirus 7 (E46+) consisting of simian virus 40 (SV40) genetic material inside an adenovirus 7 capsid has been demonstrated in several laboratories.'-3 These particles have variously been called "0)," "hybrid,"14 "transcapsidant," or "PARA."5 They are able to induce SV40 T and transplantation antigens, but not SV40 V antigen or virus. IUnderstan...

Biological signal transduction by photoactive yellow protein (PYP) in halophilic purple sulfur bacteria is initiated by trans-to-cis isomerization of the p-coumaric acid chromophore (pCa) of PYP. pCa is engaged in two short hydrogen bonds with protein residues E46 and Y42, and it is negatively charged at the phenolate oxygen. We investigated the role in the isomerization process of the E46 shor...

E46(+) virus purified by limiting dilution and plaque isolation in AGMK cells consists of a mixture of hybrid and non-hybrid virus particles. Of 102 plaque isolates obtained in HEK cells, 98 contained no detectable hybrid virus, while 4 contained a small proportion of hybrid virus. Plaque isolates from AGMK cells uniformly induced SV40 neoantigen, but when these plaque isolates were plaqued in ...

Recent studies have established that the adenovirus type 7 (Ad. 7) strain E46+ is a mixed population of infectious adenovirus particles and defective particles consisting of adenovirus capsids containing the segment of SV40 DNA which codes for T antigen.1-4 Because of the inability to obtain the SV40 DNA carriers as a pure population, it has not been possible to determine whether they also carr...

The DNA and protein components of the adenovirus 7-simian virus (SV)40 hybrid virus "E46(+)" have been investigated. The Ad.7-SV40 hybrid DNA genome could be distinguished from nonhybrid Ad. 7 genome on the basis of its molecular weight in an alkaline sucrose gradient. The hybrid genome could also be separated from nonhybrid adenovirus 2 DNA when purified transcapsidant adenovirus 2(+t7) (Ad.7-...

Collagen Triple Helix Repeat Containing-1, CTHRC1, is a 30kDa secreted protein that has the ability to inhibit collagen matrix synthesis. CTHRC1 is glycosylated and retains a signal sequence consistent with it being secreted from the cell. Activity of the protein is reliant on removal of the propeptide and while CTHRC1 does not contain a predictable consensus propeptide cleavage site, there is ...

1. Fejjal N, Belmir R, El Mazouz S, Gharib N, Abbassi A, Belmahi A. Reversed cross finger subcutaneous flap: A rapid way to cover finger defects. Indian J Plast Surg 2008;41:55-7. 2. Braga-Silva J. Anatomic basis of dorsal finger skin cover. Tech Hand Up Extrem Surg 2005;9:134-41. 3. Kawakatsu M, Ishikawa K. Dorsal digital perforator flap for reconstruction of distal dorsal finger defects. J Pl...