Yi-Chih Chien and Glen E. Collier (1997) Biochemical comparison of arginine kinase allozymes in Drosophila melanogaster. Zoological Studies 36(4): 277-287. ARKs is a rare arginine kinase allozyme found in natural populations of Drosophila melanogaster. To test whether the rarity of this allozyme could be due to its biochemical impairment relative to the common allozyme, biochemical properties s...

Uridine diphosphate glucuronosyltransferase 1A (UGT1A) is a major phase II drug-metabolism enzyme superfamily involved in the glucuronidation of endobiotics and xenobiotics in humans. Many polymorphisms in UGT1A genes are reported to inhibit or decrease UGT1A activity. In this study, two UGT1A1 allozymes, UGT1A1 wild-type and a splice mutant, as well as UGT1A9 wild-type and its three UGT1A9 all...

We have used gluconate-limited chemostats to study selective differences between isogenic strains of Escherichia coli K12 into which four naturally occurring alleles coding for allozymes of 6-phosphogluconate dehydrogenase (6PGD) had been transferred. The limit of detectability of selection with our procedures is a selection coefficient of 0.5%. In the normal E. coli K12 genetic background, all...

T HE controversial history of allozyme studies: Alternative heritable forms of enzymes, differing in charge or shape, have been known since the 1940s; these may be alleles of one gene (allozymes) or products of distinct but related genes (isozymes). LEWONTIN and HUBBY (1966), finding an unexpected bonanza of allozyme variation in Drosophila, re-cast the existing debate about the evolutionary me...

Human polymorphic N-acetyltransferase (NAT2) catalyzes the N-acetylation of arylamine carcinogens and the metabolic activation of N-hydroxyarylamine and N-hydroxyarylamide carcinogens by O- and N,O-acetylation, respectively. Rapid and slow acetylator phenotype is regulated at the NAT2 locus, and each has been associated with differential risk to certain cancers relating to carcinogenic arylamin...

The teleost Fundulus heteroclitus (L.) possesses two loci, Gpi-A and Gpi-B, for the glycolytic enzyme, glucose-phosphate isomerase (GPI; D-glucose-6-phosphate ketol-isomerase; E.C. 5.3.1.9). The Gpi-B locus is polymorphic in Fundulus, with two common alleles, Gpi-Bb and Gpi-Bc, distributed in a clinal manner in populations along the east coast of North America. Since this clinal distribution is...

Phosphoglucose isomerase (PGI) allozymes were isolated from the wing-polymorphic water strider, Limnoporus canaliculatus, and were characterized biochemically with respect to temperature-dependent kinetic and thermostability properties. At higher temperatures, the allozymes exhibited significant differences in Michaelis constant (Km) values for substrates of both the forward and reverse reactio...

enzyme electrophoresis was used to measure genetic variation within, and divergence among, three generations of recently bred synthetic alfalfa generations (syn1, syn2, and syn3) originating from a polycross of 12 selected parents and several cultivars. three isozyme loci, exhibiting tetrasomic inheritance in 10-day seedlings, were detected from five enzymatic systems analyzed by polyacryla...

High-resolution electrophoresis has revealed 10 allozymes of esterase-6 (EC 3.1.1.1) in Drosophila melanogaster. The sequences of 13 isolates of the Est6 gene covering all 10 allozymes were obtained and 52 nucleotide differences were found. Sixteen of these cause amino acid replacements, of which three result in charge differences whose size and direction are consistent with the electrophoretic...