background and objective: diagnosis of hirschsprung’s disease (hd) as the most common cause of neonatal intestinal obstruction is based on the presence of aganglionosis from seromuscular or full thickness biopsy. due to the complication of full thickness or seromuscular rectal biopsy, mucosal-sub mucosal biopsy is more intended. however, interpretation of these biopsies stained with hematoxylin...