human brucellosis is a major health problem in developing countries. a number of patients with human brucellosis do not recover from the acute stage of the disease and pass into the chronic form. since the pathophysiology of this change is not well understood, we studied the t-cell subsets during the acute, subacute and chronic forms of human brucellosis. in this study we found alterations in t...

introduction: the present study was undertaken to produce alkaline phosphatase anti- alkaline phosphatase (apaap) complex and to use it in one of the most practical methods of determining the site of antigen in tissue or in cell, i.e. apaap technique, and its comparison with similar foreign products. materials and methods: in this basic and applied study the secreted antibodies of the two hybri...

background: to investigate the effect of cana2-edta and experimental diabetes (iddm) on zinc and carbohydrate metabolism and the activities of got, gpt and alkaline phosphatase. methods: forty male normal albino (wistar) rats of 8 weeks of age were fed with a basal diet. twenty rats were then intraperitoneally injected with alloxan to induce diabetes. then after one week ten rats from each grou...

conclusions the elevation of hmw-alp activity in cu treated animal suggests the occurrence of biliary disease. this may be used as a biomarker for the diagnosis of copper toxicity. results obtained data showed that with increasing administration of copper, the alp activity was elevated significantly. in comparison with the control group the elevations were between 20%-56% using gel filtration c...

introduction: salivary calcium and phosphate levels might have a relationship with dental caries severity. evaluation of different indexes such as alkaline phosphatase, which increases salivary phosphate levels, and hydroxyapatite ion activity, which reflects salivary calcium and phosphate levels, might help determine the risk of dental caries. the aim of the present study was to compare saliva...

The FLAG peptide, AspTyrLysAspAspAspAspLys, has been used as an epitope tag in a variety of cell types. The modification of the cytomegalovirus (CMV) promoter containing vector, pCMV5, to create two transient expression vectors designed for secretion and intracellular expression of FLAG-fusion proteins in mammalian cells is described. As a functional test, the bacterial alkaline phosphatase gen...

Bile duct ligation causes a five- to sevenfold increase in the activity of rat liver alkaline phosphatase within 12 hours after ligation and a similar rise in the activity of alkaline phosphatase in serum. The increased serum activity is due entirely to the appearance of a new isoenzyme that has the properties of rat liver alkaline phosphatase. The increase in both serum and liver alkaline phos...

objective: different techniques have been suggested for the repair of bone defects at the injured sites. use of biomembranes, or application of plasma rich in growth factor (prgf) at the site of proliferation of osteoblasts are among the suggested techniques. the current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hplf) cultured on hypro-sorb f, perica...

alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide e. c. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. this enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...

introduction: hydatid cyst disease is caused by the protoscolices of echinococcus granulosus. alkaline phosphatase (alp) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. the aim of this study was to compare the level of alp activity (as a pathological biomarker) in hydatid cyst protoscolices (hcp) with that of sheep liver tissue and to determine t...