background: bone marrow mesenchymal stem cells (mscs) are one of the undifferentiated multipotential cell sources of human body. methods: mscs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. learning about responses of mscs to external milieu and chemical factors such as ph could recommend new approaches for preparation of suitable scaffolds for bone a...

Cyclic AMP content in embryonic chick pelvic cartilage increases significantly as the embryo ages from 8 to 10 d. This in ovo elevation in cyclic AMP content precedes maximal cartilage alkaline phosphatase activity by some 24 h. We studied whether this temporal relationship may be causally related, using an in vitro organ culture. Incubation of pelvic cartilage from 9- and 10-d embryos in mediu...

Rosenkranz, Herbert S. (Columbia University, New York, N.Y.) and Howard S. Carr. Lethality of streptomycin for alkaline phosphatase constitutive Escherichia coli. J. Bacteriol. 85:751-753. 1963.-An alkaline phosphatase constitutive strain of Escherichia coli was more sensitive to streptomycin in a phosphorus-rich medium than was the wild parent strain. In a low-phosphorus medium, the efficiency...

Chick embryo tibial chondrocytes release into their extracellular matrix several species of proteochondroitin sulfate and collagen as well as matrix vesicles that are rich in Ca2+ and alkaline phosphatase and that appear to play a role in the calcification of cartilage. To determine whether there was any parallel regulation of the production of these products, the rates of collagen synthesis by...

The increase in alkaline phosphatase in asynchronous cultures of HeLa S(3) cells grown in medium supplemented with hydrocortisone is characterized by a lag period of 10-12 hr. Present studies utilizing synchronous cell populations indicate: (a) a minimum of 8-10 hr of incubation with hydrocortisone is necessary for maximum induction of alkaline phosphatase; (b) the increase in enzyme activity p...