نتایج جستجو برای: alkaline comet assay

تعداد نتایج: 265321  

Amin Mirzaeian Hamideh Jafari-Ghahfarokhi Javad Saffari-Chaleshtori Marzieh Sadeghiani Mehdi Allahbakhshian-Farsani Morteza Hashemzadeh-Chaleshtori Narges Abdian Payam Ghasemi-Dehkordi

Background & Aims: Induced pluripotent stem cells (iPSCs) have the capability to undergo unlimited selfrenewal and differentiation into all cell types in the body. These cells are artificially derived from a nonpluripotent cell, typically human dermal fibroblasts (HDFs). The study of cytogenetic stability of these cells, in order to use iPS cells and apply studies in therapeutic applications, i...

Journal: :Electrophoresis 2010
Katerina Afanasieva Marianna Zazhytska Andrei Sivolob

We investigated the mechanisms of DNA exit during single-cell gel electrophoresis (the comet assay) by measuring the kinetics of the comet tail formation. In the neutral comet assay, the rate of DNA exit was found to be dependent on the topological state of DNA, which was influenced by either ethidium bromide or a low radiation dose. The results clearly show that the comet tail is formed by ext...

2017
Farideh Koohian Ahmad Shanei Daryoush Shahbazi-Gahrouei Seyed Hossein Hejazi Mohammad-Taghi Moradi

In this study, we evaluated whether the protective potential of resveratrol (RSV; 3,5,4'-trihydroxy-trans-stilbene) against γ-radiation caused damages in peripheral blood lymphocyte of mice. Resveratrol as a polyphenolic compound scavenges free radicals. Various doses of RSV were administered intraperitoneally 2 hours to adult male mice before a single dose of whole-body γ-irradiation (2 Gy). T...

Journal: :Environmental and molecular mutagenesis 2000
K J Angelis M McGuffie M Menke I Schubert

The alkylating mutagens N-methyl-N-nitrosourea (MNU) and methyl methanesulfonate (MMS) were studied for their potential to induce DNA strand breaks and abasic (AP) sites in meristematic nuclei of Vicia faba root tips by the comet assay. The alkaline unwinding/neutral electrophoresis (A/N) and alkaline unwinding/alkaline electrophoresis (A/A) protocols were used for detection of DNA damage. With...

2014
Elva I. Cortés-Gutiérrez Carmen López-Fernández José Luis Fernández Martha I. Dávila-Rodríguez Stephen D. Johnston Jaime Gosálvez

Key ConceptsThe two-dimensional Two-Tailed Comet assay (TT-comet) protocol is a valuable technique to differentiate between single-stranded (SSBs) and double-stranded DNA breaks (DSBs) on the same sperm cell.Protein lysis inherent with the TT-comet protocol accounts for differences in sperm protamine composition at a species-specific level to produce reliable visualization of sperm DNA damage.A...

Journal: :iranian journal of radiation research 0
k. rajesha nairy depatment of studies in physics, mangalore university, mangalagangotri-574 199, india n.n. bhat radiological protection and advisory division, bhabha atomic research center, mumbai-400 085, india p. joseph depatment of studies in physics, mangalore university, mangalagangotri-574 199, india g. sanjeev depatment of studies in physics, mangalore university, mangalagangotri-574 199, india n. yerol depatment of studies in physics, mangalore university, mangalagangotri-574 199, india

background: exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and dna through radiolysis of cellular water. since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the dna damage and repair kinetics would help to predict the radiation sensitivity of cel...

Journal: :Genetics and molecular research : GMR 2003
M G Martino-Roth J Viégas D M Roth

The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were analyzed for e...

Journal: :Cytometry. Part A : the journal of the International Society for Analytical Cytology 2006
Nor F Rajab Declan J McKenna Jim Diamond Kate Williamson Peter W Hamilton Valerie J McKelvey-Martin

BACKGROUND Nuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation. METHODS The alkaline Comet assay was performed on six ...

2016
Soudabeh Imanikia Francesca Galea Eszter Nagy David H. Phillips Stephen R. Stürzenbaum Volker M. Arlt

This study aimed to establish a protocol for cell dissociation from the nematode Caenorhabditis elegans (C. elegans) to assess the genotoxicity of the environmental pollutant benzo[a]pyrene (BaP) using the alkaline version of the single cell electrophoresis assay (comet assay). BaP genotoxicity was assessed in C. elegans (wild-type [WT]; N2, Bristol) after 48h exposure (0-40μM). Induction of co...

Journal: :Environmental and molecular mutagenesis 2000
R R Tice E Agurell D Anderson B Burlinson A Hartmann H Kobayashi Y Miyamae E Rojas J C Ryu Y F Sasaki

Atthe International Workshop on Genotoxicity Test Procedures (IWGTP) held in Washington, DC, March 25-26, 1999, an expert panel met to develop guidelines for the use of the single-cell gel (SCG)/Comet assay in genetic toxicology. The expert panel reached a consensus that the optimal version of the Comet assay for identifying agents with genotoxic activity was the alkaline (pH > 13) version of t...

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