1 . The quantification of alantitrypsin (alAT) by standard immunological techniques is altered by interaction of the protein with leucocyte elastase. 2. The results obtained for al-antitrypsinleucocyte elastase mixtures in the presence of a functional excess of the inhibitor were relatively accurate for the fust 6 h. However, continued incubation for more than 24 h led to a major overestimation...

Treatment of acute venous thromboembolism (VTE) and prophylaxis of recurrent events has been investigated in the THRIVE (THRombin Inhibitor in Venous Thrombe Embolism) Treatment and the THRIVE III trial using the oral direct thrombin inhibitor ximelagatran. Alanine aminotransferase (ALAT) increased in 9.6% and 6.4% of patients in the THRIVE Treatment and THRIVE III trials, respectively. The aut...

Current concepts relating to the pathogenesis of emphysema associated with cigarette smoking is that an imbalance exists within the lower respiratory tract between neutrophil elastase and the local anti-neutrophil elastase screen, enabling uninhibited neutrophil elastase to destroy the alveolar structures over time. The possible role of alveolar macrophages in contributing to this imbalance was...

Using Biochemica Test Kits (Boehringer GmbH, Mannheim) a significant difference in alanine aminotransferase : aspartate aminotransferase (ALAT : ASAT) ratios was found between Trypanosoma brucei brucei (4 strains; mean ± S.E.; 4.204 ± 0.342) and T. b. rhodesiense (4 strains; mean ± S.E.: 2.973 ± 0.174) blood¬ stream stage crude soluble enzyme extracts. This difference is a stable characteristic...

Aminotransferases (ATs) interacting with L-alanine are the least studied bacterial ATs. Whereas AlaT converts pyruvate to L-alanine in a glutamate-dependent reaction, AvtA is able to convert pyruvate to L-alanine in an L-valine-dependent manner. We show here that the wild type of Corynebacterium glutamicum with a deletion of either of the corresponding genes does not exhibit an explicit growth ...

BACKGROUND AND AIMS We aimed to study the prevalence and the predictive factors of non-alcoholic fatty liver disease (NAFLD) defined by the fatty liver index (FLI) in type 2 diabetic patients (T2DM). METHODS Three hundred and eighty-one T2DM outpatients who regularly attended a Consulting Clinic in Cluj were retrospectivelly included. FLI, a surrogate steatosis biomarker based on body mass in...

Cuvinte cheie: hepatita cronică virală, fibroza hepatică, puncţie biopsie hepatică, ALAT, ASAT Rezumat: Reacţia fibrogenică este consecinţa mecanismelor imunologice desfăşurate în prezenţa virusului care are influenţă asupra fibrogenezei. Scopul studiului a fost de a observa dacă există o legătură între modificările histologice şi valorile crescute ale ALAT şi ASAT. În acest studiu toţi pacienţ...

From experiments with purified proteins, it has been concluded that factor Xla (FXla) is inhibited in plasma mainly by a,-antitrypsin (alAT), followed by antithrombin 111 (ATIll), Cl-inhibitor (Cllnh), and a2-antiplasmin (a2AP). However, the validity of this concept has never been studied n plasma. We established the relative contribution of different inhibitors to the inactivation of FXla in h...

Cancer cells commonly exhibit increased nonoxidative D-glucose metabolism whereas induction of mitochondrial metabolism may impair malignant growth. We have first used an in silico method called elementary mode analysis to identify inhibition of ALAT (L-alanine aminotransferase) as a putative target to promote mitochondrial metabolism. We then experimentally show that two competitive inhibitors...

Three primigravidae were admitted during the first trimester of pregnancy with nausea, vomiting, ketonuria and liver enzyme elevation of varying severity. A 29-year-old woman had elevated aminotransferase values, at levels described in the literature (ASAT 112 U/l, ALAT 214 U/l). The second patient, a woman aged 26 years, had undergone in vitro fertilisation and showed higher liver enzyme eleva...