نتایج جستجو برای: agroinfiltration

تعداد نتایج: 171  

2015
Qiang Chen Huafang Lai

Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms. In this paper, we review the recent ...

2014
Juan Du Hendrik Rietman Vivianne G. A. A. Vleeshouwers

Agroinfiltration and PVX agroinfection are two efficient transient expression assays for functional analysis of candidate genes in plants. The most commonly used agent for agroinfiltration is Agrobacterium tumefaciens, a pathogen of many dicot plant species. This implies that agroinfiltration can be applied to many plant species. Here, we present our protocols and expected results when applying...

2017
Huimin Zhao Zilong Tan Xuejing Wen Yucheng Wang

Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency. Here, we studied the factors influencing the transformation efficiency of syringe agroinfiltration. The pCAMBIA1301 was transformed into Nicotiana benthamiana...

Journal: :PLoS ONE 2009
Pudota B. Bhaskar Muthusubramanian Venkateshwaran Lei Wu Jean-Michel Ané Jiming Jiang

Potato is the third most important food crop worldwide. However, genetic and genomic research of potato has lagged behind other major crops due to the autopolyploidy and highly heterozygous nature associated with the potato genome. Reliable and technically undemanding techniques are not available for functional gene assays in potato. Here we report the development of a transient gene expression...

Journal: :European Journal of Plant Pathology 2021

Crude protein fractions from Mirabilis jalapa of northern Iran containing Antiviral Protein (MAP) reduced the systemic accumulation a plant RNA virus in Nicotiana benthamiana. A MAP gene was amplified by RT-PCR total RNAs isolated these Iranian M. plants (MAP-Tehran) without 5´-end signal peptide sequence, to set up an experimental system that would transiently express cell cytosol agroinfiltra...

Hasan Marashi Maziar Habibi-Pirkoohi, Mohammadreza Nassiri, Nasrin Moshtaghi, Saeid Malekzadeh-Shafaroudi Saeid Zibaee

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

2017
Ik-Hyun Kim Jae-Yeong Han In-Sook Cho HyeKyoung Ju Jae Sun Moon Eun-Young Seo Hong Gi Kim John Hammond Hyoun-Sub Lim

The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was suc...

Journal: :Virology 2010
Hyoun-Sub Lim Anna Maria Vaira Leslie L Domier Sung Chul Lee Hong Gi Kim John Hammond

We have developed plant virus-based vectors for virus-induced gene silencing (VIGS) and protein expression, based on Alternanthera mosaic virus (AltMV), for infection of a wide range of host plants including Nicotiana benthamiana and Arabidopsis thaliana by either mechanical inoculation of in vitro transcripts or via agroinfiltration. In vivo transcripts produced by co-agroinfiltration of bacte...

Journal: :Methods in molecular biology 2015
Vinay Panwar Brent McCallum Guus Bakkeren

With the rapid growth of genomic information, there is an increasing demand for efficient analysis tools to study the function of predicted genes coded in genomes. Agroinfiltration, the delivery of gene constructs into plant cells by Agrobacterium tumefaciens infiltrated into leaves, is one such versatile, simple, and rapid technique that is increasingly used for transient gene expression assay...

Journal: :iranian journal of biotechnology 2014
maziar habibi-pirkoohi saeid malekzadeh-shafaroudi hasan marashi nasrin moshtaghi mohammadreza nassiri

background: transient and stable transformation of host plants are the common techniques to produce transgenic plants. however, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. while, transient gene expression is a quick method to produce recombinant proteins in plants.  objective: the main goal of the present study was to eval...

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