Vitamin E is one of the lipid soluble vitamins consisting of several isoforms, including tocopherols and tocotrienols amongst which the alpha tocopherol is the most active one. The conversion of γ tocophrol to α tocopherol takes place by the activity of γ-tmt enzyme. Many plants including canola lack the γ-tmt gene to enable them to convert γ tocophrol into α tocopherol. The aim of this study w...

Root nodulating bacteria was isolated from root nodules of Vigna trilobata (L) Verdc. Plants raised in the soils were collected from five geographically different areas in Andhra Pradesh, India. The bacteria was identified as Agrobacterium tumefaciens strains by 16S rDNA analysis. Except A. tumefaciens MRR 102, all the strains produced round shape and white coloured colonies with lot of mucilag...

Crown gall of walnut (Juglans sp.), caused by the bacterium Agrobacterium tumefaciens, greatly impacts the CA walnut industry. To determine the genetic diversity of A. tumefaciens throughout the Central Valley of CA, we collected isolates from ten walnut growing counties. A total of 340 A. tumefaciens biovar 1 isolates were collected and tested for the presence of the Ti plasmid by PCR; 190 iso...

BACKGROUND Agrobacterium tumefaciens strain GV3101 (pMP90) is widely used in transient gene expression assays, including assays to study pathogen effectors and plant disease resistance mechanisms. However, inoculation of A. tumefaciens GV3101 into Nicotiana tabacum (tobacco) leaves prior to infiltration with pathogenic and non-host strains of Pseudomonas syringae results in suppression of macro...

Previous studies have shown that the widely used plant transformation vector Agrobacterium tumefaciens can persist in genetically engineered plants in vitro and in transgenic greenhouse-grown plants, despite the use of counter-selective antibiotics. However, little is known regarding Agrobacterium persistence in tree species. To understand the kinetics of A. tumefaciens decline and persistence ...

Crown gall of walnut (Juglans sp.), caused by the bacterium Agrobacterium tumefaciens, greatly impacts the CA walnut industry. To determine the genetic diversity of A. tumefaciens throughout the Central Valley of CA, we collected isolates from 12 walnut growing counties. A total of 340 A. tumefaciens biovar 1 isolates were collected and tested for the presence of the Ti plasmid by PCR; 267 isol...

Many cellular processes critically depend on the membrane composition. In this review, we focus on the biosynthesis and physiological roles of membrane lipids in the plant pathogen Agrobacterium tumefaciens. The major components of A. tumefaciens membranes are the phospholipids (PLs), phosphatidylethanolamine (PE), phosphatidylglycerol, phosphatidylcholine (PC) and cardiolipin, and ornithine li...

Scientific achievement in molecular biology and genetic engineering have made it possible to create new methods of selection k that are based on genome modification. Todays, more genetically modified plants being intensively tested the field, but their influence has not yet been fully studied environment, biodiversity, human health, animal body.
 The article analyzes results research prese...

Azotobacter vinelandii stimulated the growth of Agrobacterium tumefaciens H2, H23, H24, H27, and ATCC 15955 on media containing insoluble iron sources. The Azotobacter vinelandii siderophores appeared to promote Agrobacterium tumefaciens growth by solubilizing mineral iron, and the ferrisiderophores so formed then acted as iron sources for Agrobacterium tumefaciens. Agrobactin, the Agrobacteriu...

We have sequenced the complete genome of the plant pathogen Agrobacterium tumefaciens strain LBA4213, a derivative of the wild-type strain A. tumefaciens Ach5 and the ancestor of A. tumefaciens strain LBA4404 used in genetic engineering. The genome consists of a circular chromosome and a linear chromosome, as well as a megaplasmid and a tumor-inducing plasmid.