AIMS Silver stainable nucleolar organizer regions (AgNORs) have received a great deal of attention recently as their frequency within the nuclei is significantly higher in malignant cells than in normal, reactive or benign neoplastic cells. The objective of this study was to carry out a quantitative assessment of large and small AgNORs in oral normal mucosa, precancerous lesions and infiltratin...

Argyrophilic nucleolar organizer regions (AgNORs) analysis of 20 estrogen receptor positive (ER+) and 16 estrogen receptor negative (ER-) invasive ductal breast carcinomas was performed. The quantification of AgNORs was determined by using morphometrical method in three ways: 1) mean number of AgNORs per nucleus counted on a 100 random nuclei, 2) mean number of AgNORs per nucleus counted on 200...

The present study was undertaken to provide missing information on the distribution of AgNORs in large nucleoli of human leukaemic early granulocytic precursors in vivo as well as in vitro. In vivo, the distribution of AgNORs was studied in early granulocytic precursors of patients suffering from chronic myeloid leukaemia who were both untreated and treated with imatinib mesylate. AgNORs were v...

OBJECTIVE Evaluate the relationships between AgNORs polymorphisms and squamous intraepithelial lesions (SIL) and squamous cell carcinoma (SCC) with HPV infection. MATERIALS AND METHODS A study was carried out on sixty women from the state of Guerrero, Mexico. HPV detection was performed by PCR. AgNORs were identified by argentic impregnation. One hundred cells per slide were counted and class...

To determine the changes in histologic features during the course of prostate cancer under long-term endocrine therapy, histologic grade and argyrophilic nucleolar organizer regions (AgNORs) were examined in specimens before treatment, at relapse, and at cancer death. A total of 29 patients who had received therapy and died of prostate cancer were evaluated. Among the 29 cases, biopsy tissues b...

Background: Oral cancer is one of the most significant reasons death in India. The quantitative and qualitative assessment AgNORs parameters has been used oncology for both diagnostic prognostic objectives. number distribution AgNOR nucleus (configuration) were helpful detecting predicting prognosis various neoplasias. Aim: To assess biologic aggressiveness leukoplakia, oral submucous fibrosis ...

The variations in the numbers of nucleolar organising regions (NORs) among different grades of cervical intraepithelial neoplasia (CIN) were investigated using a silver staining technique. Twenty four biopsy specimens were studied (six normal and six of each of the three grades of CIN) by staining paraffin wax sections using a silver (AgNOR) method that stains the NORS as multiple black dots wi...

Active nucleolar organizer regions (NORs) are affine to silver (Ag) and therefore visible in silver stained cell specimens. These so called AgNORs are of diagnostic relevance for early cancer diagnosis. Therefore we seek to automatically detect and segment these dark, spot-like regions. This is challenging, since the contrast between the AgNORs and the nucleus background varies heavily from cel...

STUDY OBJECTIVES To evaluate the usefulness of argyrophilic nucleolar organizer region (AgNOR) counting and flow cytometric DNA analysis in the differential diagnosis of thymoma and thymic carcinoma, as well as in the differences among various stages and histologic subtypes of these tumors. DESIGN AND INTERVENTIONS Paraffin-embedded blocks of 64 thymic epithelial tumors (20 noninvasive thymom...