Discrimination of regenerative cirrhotic nodules of the liver (CN) from hepatocellular carcinoma (HCC) is sometimes difficult. We examined the utility of AgNOR staining in this context. Fifteen cases of HCC and 25 cases of CN were stained by AgNOR method and the mean AgNOR number for one hundred nuclei in each case was determined. There was a significant difference between the mean AgNOR c...

Study of AgNOR has been identified as a reliable indicator of cell proliferation and in turn, allows a clear distinction between benign, premalignant, and malignant epithelial changes. The aim of this study was to analyze and evaluate the application of AgNOR method as diagnostic tool in non-neoplastic and neoplastic lesions of endometrium. The study included evaluation of AgNOR staining on a t...

Fifteen normal bone marrow aspirates were stained with the agyrophilic nucleolar organiser region (AgNOR) method. The results of the specific staining AgNORs as well as nuclear and cytoplasmic staining were analysed. A system was devised to characterise precisely the AgNORs present in the nuclei of bone marrow cells. Particular types of bone marrow cells had a characteristic AgNOR and non-AgNOR...

The selective staining of nucleolar acidic proteins by silver dyes (AgNOR technique) is a promising tool to discriminate malignant cells from benign cells in tumour pathology. Until now, the existing methods to measure the distribution of the AgNOR proteins were performed in two-dimensional space. Confocal scanning laser microscopy (CSLM) and progress in computer sciences make it possible to im...

Background: Nucleolar organizing regions (NORs) are associated with proliferative activity and represents as a diagnostic aid in several neoplastic entities. Objective: The purpose of this study was to determine and compare the AgNOR (Silver binding nucleolar organizing region) count in healthy oral mucosa, oral leukoplakia and oral squamous cell carcinoma (OSCC). Methods: The biopsied tissue s...

The aim of this study was to evaluate the diagnostic value of AgNOR (Argyrophilic Nucleolar Organizer Proteins Region) staining in differentiation between malignant and non-malignant lesion, and histological grading of bladder transitional cell carcinoma. We have also showed the rate of AgNOR distribution score in the nuclear of malignant and non-neoplastic cells of bladder epithelium as an imp...

After the pioneering work by Ploton et al. [1] silver staining of nucleolar organizer regions (AgNOR) has been introduced into tumour pathology as a proliferation marker which is closely related to the rapidity of cell cycle [2]. Owing to wet autoclave pretreatment introduced by Öfner et al. [3] AgNOR analysis has now widely been standardized in tissue sections [4]. In cytological specimens, ho...

The argyrophilic nucleolar organizer region (AgNOR) of 100 cancer cells from biopsy specimens of esophageal squamous cell carcinomas in 98 surgically treated cases was examined, using a silver colloid staining technique on biopsy specimens. The number of AgNOR per nucleus (AgNOR number) was higher in the more advanced groups with regard to the length of the tumor (P less than 0.01), the depth o...

The aim of the study was an evaluation of the proliferation activity of Central Nervous System tumours by using silver colloid staining of nucleolar organiser regions (AgNORs) and comparing the results with the proliferation activity estimated by using PCNA and Ki-67 antibodies to clarify the clinicopathological significance of this method. The first part of the study describes the results of A...