To assess the incidence of aflatoxins contamination in different commodities, a representative countrywide study was conducted. One hundred composed samples of cereals, grains, spices, condiments and refined and raw sugars were analysed for aflatoxin B1, B2, G1 and G2. The moisture contents of all the food samples were also invariably determined. Only 6% of the samples namely maize and red chil...

Aflatoxins are a family of toxic, acetate-derived decaketides that arise biosynthetically through polyhydroxyanthraquinone intermediates. Most studies have assumed that aflatoxin B1 is the biosynthetic precursor of the other aflatoxins. We used a strain of Aspergillus flavus which accumulates aflatoxin B2 to investigate the later stages of aflatoxin biosynthesis. This strain produced aflatoxins...

background: aflatoxins cause health hazards to human and animals and has also economical problems. therefore, the de­toxification effect of citric acid was investigated in rice as the main food of iranian people. methods: initially 275 samples of rice were examined for aflatoxins by hplc. the aflatoxins contaminated samples were later treated by aqueous citric acid and detoxification of aflatox...

Monoclonal antibodies specific for aflatoxin B1, aflatoxin B2, aflatoxin M1, and the major aflatoxin-DNA adducts were obtained following fusion of mouse SP-2 myeloma cells with spleen cells of mice immunized with aflatoxin B1 covalently bound to bovine gamma globulin. The aflatoxin-modified protein used to immunize mice was produced chemically by activating aflatoxin B1 to a 2,3-epoxide derivat...

Administration of[3H]aflatoxin B2 (2,3-dihydroaflatoxin B1)(AFB2) to male rats resulted in levels of hepatic DNA- and ribosomal(r)RNA-aflatoxin adducts that were about 1% of those for rats given [3H]aflatoxin B1(AFB1). The levels of hepatic protein-aflatoxin adducts were 35 to 70% as great for AFB2-treated as compared to AFB1-treated rats...

During 2011~2013, a total of 729 samples for 19 types of medicinal plant were collected from Seoulyekryungsi in Seoul, Korea, and investigated for the presence of aflatoxins. The samples were analyzed using immunoaffinity column cleanup and high-performance liquid chromatography coupled to a fluorescence detector after post-column derivatization. Aflatoxins were found in 124 out of the 729 anal...

In order to study the contamination of Aspergillus flavus and aflatoxin production in pistachio cultivars in the Semnan province, eight cultivars of pistachio were collected from major pistachio growing areas. Using the serial dilution method, ground pistachio kernels were inoculated on plates containing AFPA medium and incubated at 28° C. This experiment was performed using three replications ...

The metabolism of aflatoxin B2 by postmitochondrial supernatant fractions of duck, rat, mouse, and human livers was studied in an in vitro system. Duck liver had a much higher level of activity than had tissues from other species. Postmitochondrial supernatant equivalent to 0.2 g whole liver metabolized 40 to 80% of the initial substrate in 30 min, compared to less than 6% for the other species...

BACKGROUND Due to the high toxicity of aflatoxin and its effects on public health, determination of aflatoxin level in Wheat flour samples in the Golestan province, north of Iran was investigated. To examine the effect of seasonal changes, summer and winter sampling was performed with standard sampling methods. METHODS A total of 200 flour samples were collected from 25 factories. HPLC method...

Background: Aflatoxin B 1 induced hepatitis was reported to be associated with characteristic features of centrizonal scarring, hepatic venous occlusion, ductular proliferation and cholestasis, focal syncytial giant cell giant cell transformation of hepatocytes, and pericelluar fibrosis which is congruent to biopsy findings in extra hepatic biliary atresia (EHBA). Aim of Work: Is to study aflat...