results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

Front Cover In article number 2201409, Daqing Sun, Dan Ding, and co-workers propose a fluorinated supramolecular strategy for developing cancer vaccine adjuvant, of which 4RDP(F5) containing tetra-arginine (4R) dipentafluorophenylalanine (DP(F5)) balances the self-assembly, antigen affinity, transmembrane abilities. The performance 4RDP(F5)-OVA in prevention treatment tumors makes it an attract...

Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from ser...

neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. currently, there are no vaccines to prevent disease caused by strains of n.meningitidis serogroup b. the class 1 outer membrane protein (omp) has been named pora which is a cation selective transmembrane protein of 45 kda that forms trimeric pore in the meningococcal outer membrane. pora from ser...

This study presents the results of a survey safety and protective efficacy candidate vector-based vaccine for bovine tuberculosis, using an influenza vector with NS1 mutation expressing M. bovis antigens ESAT-6 TB10.4. We vaccinated Balb/c outbred mice two times at 21 days apart. Our experimental design includes immunised or without adjuvant 15% Montanide Gel. The vaccine’s was determined by bi...

Objective(s): The porins A and B and also outer membrane vesicles (OMVs) of Neisseria meningitidis are used for vaccine purposes. In the present study, we aimed to design a new vaccine candidate based on a fusion of PorA of serogroups A and B of N. meningitidis admixed with OMV and evaluate it in an animal model.Materials and Methods: Af...

In recent years, heterologous prime-boost vaccines have been demonstrated to be an effective strategy for generating protective immunity, consisting of both humoral and cell-mediated immune responses against a variety of pathogens including HIV-1. Previous reports of preclinical and clinical studies have shown the enhanced immunogenicity of viral vector or DNA vaccination followed by heterologo...