A medium for detecting colonies of Actinomyces viscosus and Actinomyces naeslundii in dental plaque samples was developed. The medium (CNAC-20) contains 20.0 mug of 3CdSO4-8H2O per ml of Columbia CNA agar base. Laboratory strains of A. viscosus grew on CNAC-20 in characteristic round, white, smooth, opaque colonies. Increasing the cadmium concentration impaired the growth of some A. viscosus st...

Human dental plaque samples were screened for the presence of bacteriophage for Actinomyces viscosus and Streptococcus sanguis. None of the 336 samples yielded phage for S. sanguis, but 10 contained virulent actinomyces phage. A high host cell specificity was observed in that one phage isolate infected only A. viscosus T14V, eight phage isolates infected only A. viscosus MG-1, and one infected ...

Interbacterial adherence was sought between strains of Actinomyces viscosus indigenous to the human mouth and strains of Streptococcus pyogenes, Streptococcus agalactiae, and Pseudomonas aeruginosa. Six of nine strains of S. pyogenes, three of five strains of S. agalactiae, and two of four strains of P. aeruginosa were found to coaggregate with each of five strains of A. viscosus tested. Some c...

A lytic bacteriophage for Actinomyces viscosus T14V (the reference strain for actinomyces coaggregation group A) was isolated from raw sewage. This phage, designated BF307, also lysed the T14V-derived nonfimbriated mutant PK455-2 as well as A. viscosus MG-1 and T14AV but not the other serotype 2 or serotype 1 strains of this species that were tested or any of nine Actinomyces naeslundii isolate...

Actinomyces odontolyticus and A. viscosus are designated as group E and group F in the serological grouping of the Actinomyces.

Purified cell walls of Actinomyces viscosus and Actinomyces naeslundii were solubilized by the enzymatic action of Ml N-acetylmuramidase. Soluble cell wall carbohydrate antigens were purified by column chromatography. A purified antigen preparation (fraction C) from A. viscosus ATCC 19246 was found to contain deoxyhexoses (rhamnose and 6-deoxytalose), hexosse (galactose, glucose and mannose), a...

The isolation of Actinomyces viscosus from two patients is described. One was a case of multiple myeloma, the organism being found on blood culture; the other was a patient with a submandibular abscess. These are believed to be the first such isolations of A. viscosus in this country.

Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial...

Phagocytosis of Actinomyces viscosus T14V and A. naeslundii WVU45 by human polymorphonuclear leukocytes in the absence of antibody or complement was mediated by the lectin associated with the type 2 fimbriae of these bacteria. This effect was markedly enhanced by exogenous sialidase, an enzyme also secreted by these actinomyces. Since sialidase treatment of the bacteria did not result in increa...

this paper presents a case-report of cutaneous actinomycosis due to actinomyces viscosus in a 22 year old man, hospital staff from tehran with multiple fistulous tracts in his right hand and a history of skin injury due to thorn of plant. diagnosis was based on the observation of gram positive coccoid, diphtheroid and long branching filaments in direct examination of pus from fistulous tracts a...