background: trichomonas vaginalis is the agent of urogenital tract infection that causes human trichomoniasis with some serious health complications. more understanding about genetic features of the parasite can be helpful in the study of the pathogenesis, drug susceptibility and epidemiology of the infection. for this end,we conducted analysis of the actin gene of t. vaginalis by applying the ...

Introduction: Beta-actin is a housekeeping gene, which is used as an internal standard in various biological studies. Recently, it was reported that the expression of commonly used housekeeping genes including ß–actin changes in disease states or under certain experimental conditions. Based on the reports about the effects of sex hormones on the expression of the ß- actin gene, in this study...

background: the aim of this study was to identify the trichomonas vaginalis strains/haplotypes based on identifying their probable variations in asymptomatic patients referred to tabriz health centers, northwestern iran. methods: sampling was taken from 50-suspected women to t. vaginalis in northwestern iran. the obtained samples were smeared and cultured. fifty dna samples were extracted, ampl...

Background Bone marrow and adipose tissue are two main sources of mesenchymal stem cells (MSCs). Some of studies suggest that there are some differences in gene expression profile of MSCs-derived from various tissues. To investigate gene expression profile by qRT-PCR, an appropriate reference gene with stable expression level should be chosen for normalizing data.  This study was designed to e...

background bone marrow and adipose tissue are two main sources of mesenchymal stem cells (mscs). some of studies suggest that there are some differences in gene expression profile of mscs-derived from various tissues. to investigate gene expression profile by qrt-pcr, an appropriate reference gene with stable expression level should be chosen for normalizing data.  this study was designed to ev...

Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...

Plasma membrane H+-ATPase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. To study the effect of NaCl on the expression pattern of a gene encoding the plasma membrane H+-ATPase, an experiment was carried out in a completely random design with three replications. A pair of specific primers was designed based on the sequence o...

Background & Aims: Atherosclerosis is a major cause of morbidity and mortality. Adiponectin reducesthe risk of heart disease, and matrix metalloproteinase-9 (MMP-9) is involved in the formation and development of atherosclerotic plaque. The aim of this study was the investigation of the effect of adiponectin on MMP-9 gene expression. It seems this hormone can reduce the risk of atherosclerosis ...

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

background: in spite of sufficient knowledge about phenotypic variation of trichomonas vaginalis , its genetic characteristics are poorly understood. we carried out a molecular epidemiology study in which in vitro metronidazole susceptibility of t. vaginalis isolates was considered. methods : this study was conducted on 862 women admitted to gynecology clinics in hamadan, west of iran, during 2...