background: inflammatory bowel disease unclassified (ibdu) is considered to be an aberrant immune response with loss of tolerance to many antigens. objective: this paper tries to address whether there is any value to test for auto-antibodies in such patients. methods: 60 patients with inflammatory bowel disease unclassified participated in the study. auto-antibodies to nuclear antigen, intestin...

the histological analysis, disposition and histochemical localization of tryptophan were investigated in the pancreas to compare the cellular organization and histochemical characterization in the pancreas of labeo rohita (hamilton, 1822), mystus vittatus (bloch, 1790) and notopterus notopterus (pallas, 1769) having different feeding habits. histological analysis demonstrated that the exocrine ...

The regenerative medicine field is expanding with great successes in laboratory and preclinical settings. Pancreatic acinar cells in diabetic mice were recently converted into β-cells by treatment with ciliary neurotrophic factor (CNTF) and epidermal growth factor (EGF). This suggests that human acinar cells might become a cornerstone for diabetes cell therapy in the future, if they can also be...

INTRODUCTION The methodology of acinar cell culture has become of primary importance in the research of pancreatic physiology and pharmacology. AIM To develop a method for primary culture of porcine pancreatic acinar cells. METHODOLOGY Dispersed pancreatic acinar cells were made by RPMI-1640 medium containing collagenase III. After purification, the isolated acinar cells were cultured in RP...

A promising approach to new diabetes therapies is to generate β cells from other differentiated pancreatic cells in vivo. Because the acinar cells represent the most abundant cell type in the pancreas, an attractive possibility is to reprogram acinar cells into β cells. The transcription factor Pdx1 (Pancreas/duodenum homeobox protein 1) is essential for pancreatic development and cell lineage ...

In addition to causing necrosis of /?-cells, diabetogenic doses of alloxan or streptozotocin cause selective autophagy and destruction of /^-granules in intermediate cells in the rat pancreas, thus providing evidence that these granules and those in the /?-cells of the islet are identical. In acinar-/? cells the autophagic vacuoles also contain small mitochondria similar to those that occur in ...

It has been suggested that pancreatic acinar cells can serve as progenitors for pancreatic islets, a concept with substantial implications for therapeutic efforts to increase insulin-producing beta cell mass in patients with diabetes. We report what we believe to be the first in vivo lineage tracing approach to determine the plasticity potential of pancreatic acinar cells. We developed an acina...

Acinar cells play an essential role in the secretory function of exocrine organs. Despite this requirement, how acinar cells are generated during organogenesis is unclear. Using the acini-ductal network of the developing human and murine salivary gland, we demonstrate an unexpected role for SOX2 and parasympathetic nerves in generating the acinar lineage that has broad implications for epitheli...

Current dogma suggests that salivary gland homeostasis is stem cell dependent. However, the extent of stem cell contribution to salivary gland maintenance has not been determined. We investigated acinar cell replacement during homeostasis, growth, and regeneration, using an inducible CreER(T2) expressed under the control of the Mist1 gene locus. Genetic labeling, followed by a chase period, sho...