objective the aim of this study was to compare the sensitivity and specificity of acid fast and auramine-rhodamine staining and multiplex pcr for the detection of mycobacterium tuberculosiscomplex and non tuberculosis mycobacteria on formalin fixed paraffin embedded tissues (ffpe) materials and methods forty cases of ffpe pleural and bronchial tissue with chronic granulomatous inflammation and ...

Case: Isolated talus osteomyelitis caused by Koch's bacillus (BK) is extremely rare. In this paper we report an isolated talus bone tuberculosis in a 52 year-old man who presented a 2-month history of swelling, pain and functional disability of his right ankle. Surgical bone curetting and histological examination showed granuloma with central caseating necrosis. Acid fast stain and PCR examin...

case: isolated talus osteomyelitis caused by koch's bacillus (bk) is extremely rare. in this paper we report an isolated talus bone tuberculosis in a 52 year-old man who presented a 2-month history of swelling, pain and functional disability of his right ankle. surgical bone curetting and histological examination showed granuloma with central caseating necrosis. acid fast stain and pcr exa...

Acid-fast staining has been utilized in the field of medicine since late 1800s. It was, for a while, considered gold standard diagnosing leprosy and tuberculosis. Principally it uses 1 primary agent, carbol fuchsin; methylene blue as counter stain. The emergence molecular-based diagnostic tests makes some us question relevance acid-fast daily practice. This literature review aimed to revisit me...

Newly available assays offer alternatives to conventional microscopic examination for Cryptosporidium spp. We compared two enzyme immunoassays, ProSpect Cryptosporidium microtiter assay (Alexon, Inc., Mountain View, Calif.) and Color Vue Cryptosporidium assay (Serady, Indianapolis, Ind.), and a direct immunofluorescent assay, Merifluor Cryptosporidium kit (Meridian Diagnostics, Cincinnati, Ohio...

Dear editor,I read with interest article that published entitled *Isolation and identification of bioactive compound producing Rhodococcus spp. isolated from soil samples {IJMCM/5(1) (2015) 463-468}* [1]. Some of the genus such as Nocardia, Gordonia, Mycobacterium and Rhodococcus are in actinomycete family and they are Gram-positive and partially acid-fast. Rhodococcus species usually stain Gra...

Histoid Leprosy (HL) is a rare variant of Lepromatous Leprosy, occurring in long-standing cases, mostly in a background of acquired drug resistance. Patients usually present with sudden onset multiple nodules and plaques, most often involving the skin and subcutaneous tissue of trunk and lower limbs. Here we report an unusual case of de novo (without any history of prior anti-leprotic therapy) ...