Wheat is the world’s largest volume crop traded internationally. Foliar diseases are the main biotic restriction reducing yield in wheat crops. Wheat productivity can be enhanced through controlling foliar disease through the use of resistant cultivars. Plant breeders are providing considerable attention to wheat genetic improvement from the past few years to minimize losses due to pests and pa...

AIMS The objective of this study was to determine if acetosyringone affected the expression of aflatoxin biosynthetic genes. METHODS AND RESULTS Two genes, nor1 and ver1, representing genes whose products are involved in early and late steps in aflatoxin biosynthesis, were examined. Two GUS (beta-glucuronidase) reporter constructs, nor1:GUS (pGAP12) and ver1:GUS (pGAP13), were used to study t...

The phenolic compounds acetosyringone, syringaldehyde and sinapinic acid inhibited the biosynthesis of aflatoxin B1 (AFB1) by A. flavus. Acetosyringone was the most active among the three compounds, inhibiting aflatoxin level by 82% at 2 m moll-1. The synthesis and accumulation of norsolorinic acid, an aflatoxin biosynthetic intermediate, was also inhibited. These results suggest that at least ...

In the present study high efficiency Agrobacterium tumefaciens mediated transformation system of wheat (Triticum aestivum L.) cv. Inqilab-91 was developed by exploiting bacterial culture density and acetosyringone concentration. Agrobacterium strain EHA101 harboring binary vector pIG121Hm, containing gene for GUS activity and hygromycin resistance was used in transformation experiments. Differe...

An efficient Agrobacterium-mediated durum wheat transformation system has been developed for the production of 121 independent transgenic lines. This improved system used Agrobacterium strain AGL1 containing the superbinary pGreen/pSoup vector system and durum wheat cv Stewart as the recipient plant. Acetosyringone at 400 microM was added to both the inoculation and cultivation medium, and picl...

An efficient Agrobacterium-mediate transformation method was developed by employing different duration of sonication, Agrobacterium strains, type of inoculation medium and concentrations of acetosyringone in Arya cultivar of wheat. Immature embryos were used as an explant for inoculation with Agrobacterium tumefaciens harboring the recombinant pBI121 plasmid. Among the durations of sonication t...

We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing β-g...

An effective transformation protocol for Dunaliella, a β-carotene producer, was developed using the synergistic mechanism of D-glucose and Acetosyringone on three different Agrobacterium strains (EHA105, GV3101 and LBA4404). In the present study, we investigated the pre-induction of Agrobacterium strains harboring pMDC45 binary vector in TAP media at varying concentrations of D-glucose (5 mM, 1...

An efficient and reproducible protocol for Agrobacteriummediated transformation of an agronomically useful Oryza sativa variety, Pusa Basmati1 (IET10364) has been standardized. Although several reporters have been claimed the Agrobacteriummediated transformation but here we studied the effect of acetosyringone and age of callus on transformation frequency for scutellumderived callus of Pusa bas...