Background and Aims: Detection of overexpression in tumor-inhibiting genes provides valuable information for leukemia diagnosis and prognosis. Chronic myeloid leukemia (CML) is a stem cell disorder determined by a well-defined genetic anomaly involving BCR-ABL translocation in the Philadelphia chromosome. Curcumin is a chemo-preventive agent for the primary cancer targets, such as the breast, p...

Background: A specific chromosomal abnormality, the Philadelphia chromosome (BCR-ABL fusion), is present in all patients with chronic myeloid leukemia (CML). The b2a2 and b3a2 fusion mRNAs encode p210 fusion protein p210 and e1a2 encode p190. The aim of this study was to evaluate the frequency of BCR-ABL fusion transcript variants in Northeast of Iranian CML patients and to compare the laborato...

Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...

abetalipoproteinemia (abl) is a very rare autosomal recessive disorder caused by mutations in the microsomal triglyceride transfer protein gene (mttp). abl is characterized by lack of lipids and apolipoprotein b (apob) in plasma, fat malabsorption and various clinical manifestations. we describe a 12-month-old infant boy, born from consanguineous parents and presented with diarrhea, steatorrhea...

It is well known that cities increase air and surface temperatures compared to their rural surroundings, the so-called urban heat island (UHI) effect. However, associated changes in atmospheric humidity (also as dry island, UDI) convection triggering remain largely unexplored it still unclear how modifications of energy budget influence diurnal evolution temperature Atmospheric Boundary Layer (...

Philadelphia (Ph')-positive acute lymphoblastic leukemia (ALL) is highly associated with two forms of chimeric bcr-abl proteins: P190bcr-abl and P210bcr-abl. Whereas P210bcr-abl also occurs in chronic myeloid leukemia, P190bcr-abl is uniquely expressed in Ph'-positive ALL. As a consequence, P190bcr-abl is preeminently a tumor-specific marker in leukemic cells of ALL patients. Because P190bcr-ab...

Allosteric inhibitors of Abl kinase are being explored in the clinic, often combination with ATP-site kinase. However, there conflicting data on whether both ATP-competitive and myristoyl-site allosteric can simultaneously bind Here, we determine is synergy or antagonism between Abl. We observe that clinical not synergistic ABL inhibitors, however, conformation-selective modulate global conform...

Small interfering RNAs (siRNAs) were designed to target the bcr-abl oncogene, which causes chronic myeloid leukemia (CML) and bcr-abl-positive acute lymphoblastic leukemia (ALL). Chemically synthesized anti-bcr-abl siRNAs were selected using reporter gene constructs and were found to reduce bcr-abl mRNA up to 87% in bcr-abl-positive cell lines and in primary cells from CML patients. This mRNA r...

c-Abl tyrosine kinase is under rigorous control because of an unknown cellular inhibitor that maintains c-Abl in a relatively inactive state. Because SH2 domains are positive regulators of the nonreceptor tyrosine kinases, we tested whether this putative inhibitor would bind to an Abl SH2 protein construct and thus activate the c-Abl tyrosine kinase. Expression of a Mr 10,000 Abl SH2 protein in...