Genomic relationships and diversity of 37 wild and cultivated wheat (Triticum sp.) possessing A genome include four T. urartu (Au), thirteen wild einkorn (Am), four cultivated einkorn (Am), seven durum wheat (BBAuAu), three T. zhukovskyi (AtAtAmAmGG) and six com...

Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...

Background: During male germ cells differentiation, genomewide re-organizations and highly specific programming of the male genome occur. These changes not only include the large-scale meiotic shuffling of genes, taking place in spermatocytes, but also a complete “re-packaging” of the male genome in post meiotic cells, leading to a highly compacted nucleo-protamine structure in the mature sperm...

background: phic31 integrase is a dna site-specific recombinase integrates dna into the chromosomes between the two sites of attb and attp. several pseudo attps have been identified in mammalian genomes with critical features for long-term expression of transgene. in this manuscript, we report a novel intrinsic pseudo attp site named chol1 in the chinese hamster genome implementing an inverse p...

The gap between the amount of genome information released by genome sequencing projects and our knowledge about the proteins' functions is rapidly increasing. To fill this gap, various 'genomic-context' methods have been proposed that exploit sequenced genomes to predict the functions of the encoded proteins. One class of methods, phylogenetic profiling, predicts protein function by correlating...

growing amount of information on biological sequences has made application of statistical approaches necessary for modeling and estimation of their functions. in this paper, sensitivity and specificity of the first and second markov chains for prediction of genes was evaluated using the complete double stranded  dna virus. there were two approaches for prediction of each markov model parameter,...

Genome-wide evaluation uses the associations of a large number of single nucleotide polymorphism (SNP) markers across the whole genome and then combines the statistical methods with genomic data to predict the genetic values. Genomic predictions relieson linkage disequilibrium (LD) between genetic markers and quantitative trait loci (QTL) in a population. Methods that use all markers simultaneo...

genomic signal processing is a relatively new field in bioinformatics, in which signal processing algorithms and methods are used to study functional structures in the dna. an appropriate mapping of the dna sequence into one or more numerical sequences enables the use of many digital signal processing tools in the analysis of different genomic sequences. also, a novel influenza a (h1n1) virus o...