نتایج جستجو برای: Pichiapastoris
تعداد نتایج: 20 فیلتر نتایج به سال:
State-of-the-art monoclonal antibody (mAb) discovery methods that utilize surface display techniques in prokaryotic and eukaryotic cells require multiple steps of reformatting and switching of hosts to transition from display to expression. This results in a separation between antibody affinity maturation and full-length mAb production platforms. Here, we report for the first time, a method in ...
leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. several approaches toward vaccine development against this disease have been taken. glycoprotein (gp63) is conserved among diverse species of leishmania and has induced immunological responses in murine models. therefore, this glycoprotein has been considered as a second generat...
background: over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. objectives: the purpose of this study was to express and purify recombinant human galect...
Background There are indications, according to WHO, that diabetes will reach 333 million people in 2025, and already is among one of the main death causes in the world. In 2030 in Latin America, diabetes mellitus will be the second cause of deaths [1]. The actions to increase the production of insulin are strategic to respond the high demands for what we have to face in the near future [2]. P. ...
Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site
Pichiapastoris is one of the most widely used expression systems for the production of recombinant secretory proteins. Its universal application is, however, somewhat hampered by its unpredictable yields for different heterologous proteins, which is now believed to be caused in part by their varied efficiencies to traffic through the host secretion machinery. The yeast endoprotease Kex2 removes...
APPLIED BIOLOGICAL SCIENCES PNAS is publishing an Editorial Expression of Concern regarding the following article: “Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichiapastoris,” by Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David H. Kung, Huijuan Li, Robert G. Miele, Juergen H. Nett, Stefan Wildt, and Tillman U. Gerngross...
APPLIED BIOLOGICAL SCIENCES PNAS is publishing an Editorial Expression of Concern regarding the following article: “Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichiapastoris,” by Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David H. Kung, Huijuan Li, Robert G. Miele, Juergen H. Nett, Stefan Wildt, and Tillman U. Gerngross...
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