نتایج جستجو برای: Livak method

تعداد نتایج: 1630094  

2016
Jolanta Góral-Półrola Małgorzata Zychowska Artur Ziółkowski Maria Pąchalska

The aim of this study was to assess the expression of genes associated with cell stress response in the patient with chronic stuttering. We examined genes expression in a 26-year-old patient (D.K.) with chronic perseverative stuttering (CPS) syndrome, the symptoms of which intensified after repeated failure to find a job and break-up with his fiancée. The symptoms of stuttering were tested usin...

Journal: :BioTechniques 2000
C A Koch-Paiz R Momenan S A Amundson E Lamoreaux A J Fornace

1.Battaglia, M., P. Pedrazzoli, B. Palermo, A. Lanza, F. Bertolini, N. Gibelli, G.A. Da Prada, A. Zambelli et al. 1998. Epithelial tumour cell detection and the unsolved problems of nested RT-PCR: a new sensitive one step method without false positive results. Bone Marrow Transplant 22:693-698. 2.Collins, C., J.M. Rommens, D. Kowbel, T. Godfrey, M. Tanner, S.I. Hwang, D. Polikoff, G. Nonet et a...

Journal: :Jurnal Ilmu Kesehatan Indonesia 2023

Latar Belakang: Kanker payudara merupakan jenis kanker dengan angka kejadiannya menempati posisi pertama terbanyak di Indonesia dibandingkan lain. Daun sirsak yang mengandung acetogenin memiliki efek sitotoksik terhadap sel T47D.
 Objektif: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun level ekspresi gen p53 T47D secara in vitro.
 Metode: penelitian eksperimental met...

2003
Thomas Mueller Alfons Gegenhuber Werner Poelz Meinhard Haltmayer

Leukemia 2001;15:1033–7. 14. Higuchi R, Fockler C, Dollinger G, Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 1993;11:1026– 30. 15. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6:986–94. 16. Soong R, Beyser K, Basten O, Kalbe A, Rueschoff J, Tabiti K. Quantitative reverse transcription-polymerase chai...

2012
Luka BOLHA Mojca NARAT Irena OVEN

1 Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia and rapid quantification results (Pfaffl, 2001; Yuan et al., 2006). Because of the lacking consensus on how to best perform qPCR, MIQE guidelines have been developed to uniform qPCR experiment setup, optimization and data analysis, making the protocols comparable between different research gro...

2016
Naveen Ramalingam Brian Fowler Lukasz Szpankowski Anne A. Leyrat Kyle Hukari Myo Thu Maung Wiganda Yorza Michael Norris Chris Cesar Joe Shuga Michael L. Gonzales Chad D. Sanada Xiaohui Wang Rudy Yeung Win Hwang Justin Axsom Naga Sai Gopi Krishna Devaraju Ninez Delos Angeles Cassandra Greene Ming-Fang Zhou Eng-Seng Ong Chang-Chee Poh Marcos Lam Henry Choi Zaw Htoo Leo Lee Chee-Sing Chin Zhong-Wei Shen Chong T. Lu Ilona Holcomb Aik Ooi Craig Stolarczyk Tony Shuga Kenneth J. Livak Marc Unger Jay A. A. West

Citation: Ramalingam N, Fowler B, Szpankowski L, Leyrat AA, Hukari K, Maung MT, Yorza W, Norris M, Cesar C, Shuga J, Gonzales ML, Sanada CD, Wang X, Yeung R, Hwang W, Axsom J, Devaraju NSGK, Angeles ND, Greene C, Zhou M-F, Ong E-S, Poh C-C, Lam M, Choi H, Htoo Z, Lee L, Chin C-S, Shen Z-W, Lu CT, Holcomb I, Ooi A, Stolarczyk C, Shuga T, Livak KJ, Unger M and West JAA (2016) Fluidic Logic Used i...

2012
Bahman Navidshad Juan Boo Liang Mohammad Faseleh Jahromi

The applications of conventional culture-dependent assays to quantify bacteria populations are limited by their dependence on the inconsistent success of the different culture-steps involved. In addition, some bacteria can be pathogenic or a source of endotoxins and pose a health risk to the researchers. Bacterial quantification based on the real-time PCR method can overcome the above-mentioned...

Journal: :Clinical chemistry 2006
Giuseppe Lippi Antonella Bassi Giorgio Brocco Martina Montagnana Gian Luca Salvagno Gian Cesare Guidi

Anticancer Res 2003;23:3327–31. 4. Weikert S, Christoph F, Schrader M, Krause H, Miller K, Muller M. Quantitative analysis of survivin mRNA expression in urine and tumor tissue of bladder cancer patients and its potential relevance for disease detection and prognosis. Int J Cancer 2005;116:100–4. 5. Schultz IJ, Kiemeney LA, Karthaus HF, Witjes JA, Willems JL, Swinkels DW, et al. Survivin mRNA c...

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