The galactose specific lectin LecA partly mediates the formation of antibiotic resistant biofilms by Pseudomonas aeruginosa, an opportunistic pathogen causing lethal airways infections in immunocompromised and cystic fibrosis patients, suggesting that preventing LecA binding to natural saccharides might provide new opportunities for treatment. Here 8-fold (G3) and 16-fold (G4) galactosylated an...

BACKGROUND Water filtration units have been faced problems in water turbidity removal related to their media, which is determined by qualitative indices. Moreover, Current qualitative indices such as turbidity and escaping particle number could not precisely determine the efficiency of the media in water filtration, so defining new indices is essential. In this study, the efficiency of Anthraci...

The Gal/GalNAc lectin is a candidate vaccine antigen for an amebiasis vaccine due to its mediation of parasite adherence to the human intestine, because partial immunity in humans is associated with a mucosal IgA response against it, and because it is effective as a vaccine against amebic colitis in the murine model. The LecA domain of the Gal/GalNAc lectin contains neutralizing antibody epitop...

Glycosphingolipids are important structural constituents of cellular membranes. They are involved in the formation of nanodomains ("lipid rafts"), which serve as important signaling platforms. Invasive bacterial pathogens exploit these signaling domains to trigger actin polymerization for the bending of the plasma membrane and the engulfment of the bacterium--a key process in bacterial uptake. ...

BACKGROUND There is growing interest in the natural and constructed wetlands for wastewater treatment. While nutrient removal in wetlands has been extensively investigated, information regarding the degradation of the pharmaceuticals and personal care products (PPCPs) has only recently been emerging. PPCPs are widely distributed in urban wastewaters and can be removed to some extent by the cons...

Natural, H2O2 and MgCl2 modified Light Weight Expanded Clay Aggregate (LECA) were used as fluoride adsorbents. Characterization of LECA and its modified forms was done by infra-red, X-ray diffraction, scanning electron microscope and X-ray fluorescence studies. The specific surface area of HML and MGML was 3.34 and 3.97 times greater than that of NL (11.72 m/g). Improved chemical composition of...

In Pseudomonas aeruginosa, many exoproduct virulence determinants are regulated via a hierarchical quorum-sensing cascade involving the transcriptional regulators LasR and RhlR and their cognate activators, N-(3-oxododecanoyl)-L-homoserine lactone (3O-C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL). In this paper, we demonstrate that the cytotoxic lectins PA-IL and PA-IIL are regulated vi...

LecA and LecB tetrameric lectins take part in oligosaccharide-mediated adhesion-processes of Pseudomonas aeruginosa. Glycomimetics have been designed to block these interactions. The great versatility of P. aeruginosa suggests that the range of application of these glycomimetics could be restricted to genotypes with particular lectin types. The likelihood of having genomic and genetic changes i...

Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternat...

Die beiden tetravalenten Lektine LecA und LecB des ESKAPE-Erregers Pseudomonas aeruginosa sind notwendig für dessen bakterielle Adhäsion, Biofilmbildung Invasion in Wirtszellen. Sie gelten daher als Ansatzpunkte zur medikamentösen antiinfektiösen Therapie. Vor kurzem berichteten wir über hochwirksame divalente Galaktoside spezifische LecA-Inhibitoren. Diese litten jedoch unter einer sehr gering...