rapd markers were employed to assess the genetic diversity of 34 iranian wild edible asparagus (a. officinalis) genotypes as well as cv. mary washington and a. persicus. in total, 80 rapd primers were employed for pcr reactions, among which, 18 revealed reasonable polymorphism. the 18 primers produced 175 bands, of which, 160 were polymorphic (91.4% polymorphism). cluster analysis of the genoty...

Edwardsiella tarda is an enterobacterial fish pathogen that causes mortality in various fish species worldwide. In this study, we analyzed the intraspecific variability in a collection of E. tarda strains isolated from turbot. To do this we employed 4 polymerase chain reaction (PCR)-based methods: (1) random amplified polymorphic DNA (RAPD), (2) enterobacterial repetitive intergenic consensus-P...

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Developme...

A total of 65 blood samples collected from Holstein cattle were employed for DNA extraction. Genomic DNA were amplified by means of random amplified polymorphic DNA (RAPD). One hundred and one random primers (Operon kits OPAA, OPAO, OPAV, OPC, OPE and OPA-06) were used for polymerase chain reactions (PCR). The PCR products were size fractionated by means of electrophoresis in agarose gel, trans...

PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discrim...

RAPD-PCR genetic markers were used to assess variation in wheat plants and connections among six genotypes. Four random primers produced 140 DNA fragments, averaging 6.7 identifiable bands per primer. Among the genotypes, 85 pieces (44.64 percent) polymorphic. Several RAPD marker had distinct signify recurrence patterns that thing differently amongst germplasm of groupings. Within-community acc...

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the cons...

rapd markers were employed to determine the diversity among 29 genotypes and cultivars of sour and duke cherry as well as a mahaleb tree genotype as an outgroup. a number of 100 random primers were examined for pcr reactions on the template dnas extracted from leaves, of which 17 primers produced polymorphic bands. these 17 selected rapd primers produced 233 bands, among which, 214 were polymor...