introduction idiopathic membranous nephropathy (mn) is a common cause of adult nephrotic syndrome. recently, m-type phospholipase a2 receptor (pla2-r) has been discovered as the main podocyte antigen in the pathogenesis of idiopathic mn. materials and methods in this mini review, the author searched english-language medline for the terms “membranous nephropathy”, “rituximab”, and “phospholipase...

objective: the study of physiological changes in recombinant cell lines provides useful information to improve production performance. in this study, we investigate the effects of an anti-cd33 chimeric igg4 expression on sp2.0 cell growth. methods: variable region genes of light and heavy chains of monoclonal antibody produced by m195 were cloned in pfuse-clig-hk and pfuse-chig-hg4 expression ...

the manipulation of redox potential in secretory pathway by thiol reducing agents can be a strategy to improve the production levels of disulfide-bonded proteins including recombinant antibodies. here we have studied the influence of cysteamine on viability and the production level of igg4 in sp2.0 cells. for this purpose, the recombinant sp2.0 cells producing an anti cd33 igg4, were subjected ...

The heavy- and light-chain variable regions from a murine monoclonal antibody that recognize Pseudomonas aeruginosa serogroup O6 lipopolysaccharide (LPS) were used to generate a series of chimeric mouse-human monoclonal antibodies with identical variable regions. The murine variable-region gene segments were cloned into an immunoglobulin (Ig) cDNA expression vector that contained the human kapp...

BACKGROUND Receptor occupancy, or saturation, assays are often utilized in preclinical and clinical development programs to evaluate the binding of a biologic to a cellular target. These assays provide critical information regarding the dose of drug required to "saturate" the target as well as important pharmacodymamic (PD) data. A flow cytometric method was developed to measure the degree of S...

Chimeric antibodies were constructed in which the murine variable region of anti-colorectal cancer monoclonal antibody CO17-1A was joined with human gamma 1, gamma 2, gamma 3, and gamma 4 constant regions. Human-mouse chimeric proteins were compared with the parental murine IgG2a antibody CO17-1A for their ability to participate in tumor-cell destruction by human and murine effector cells in an...

Serum IgG subclass levels were measured using an indirect immunoenzymatic assay (ELISA) with monoclonal antibodies in 16 children with asthma and 13 children with atopy who had mostly recurrent infections. Seven of the asthmatic children had marked low or low normal levels of IgG4, six had marked low or low normal levels of IgG 3, two had marked low normal levels of both IgG2, and IgG 3 and...

BACKGROUND Allergen-specific IgE and IgG antibodies play pivotal roles in the induction and progression of allergic hypersensitivity reactions. Consequently, monoclonal human IgE and IgG4 antibodies with defined specificity for allergens should be useful in allergy research and diagnostic tests. We used combinatorial antibody libraries and subsequent recombinant production to make and assess Ig...