Acquired resistance to the p.o. active lipophilic platinum drug bis-acetato-ammine-dichloro-cyclohexylamine platinum (i.v.) (JM216) was generated in the 41M and CH1 human ovarian carcinoma cell lines, and their resistance mechanisms were compared to parallel cisplatin-resistant (cisR) cell lines. Intracellular platinum accumulation was not reduced in either 41M/JM216R or CH1/JM216R compared to ...

We have compared the cellular accumulation and cytotoxicity of three platinum compounds in a panel of five human ovarian carcinoma cell lines. The cell lines, which were established from both untreated and pretreated patients, showed a wide range in sensitivity to cisplatin and other platinum drugs. The panel consisted of two sensitive (41M, CH1), one in vivo acquired resistant (PXN/94) with mo...

Acquired resistance to cisplatin has been generated in vitro in two human ovarian carcinoma cell lines: 41M, established from a previously untreated patient; and CH1, from a patient previously treated with cisplatin and cis-diammine-1,1-cyclobutane dicarboxylatoplatinum(II) (carboplatin). In neither cell line with acquired resistance did intracellular detoxification (via increased glutathione o...

We have compared the cellular accumulation and Cytotoxicity of three platinum compounds in a panel of five human ovarian carcinoma cell lines. The cell lines, which were established from both untreated and pretreated patients, showed a wide range in sensitivity to cisplatin and other platinum drugs. The panel consisted of two sensitive (41M, CHI), one in vivo acquired resistant (PXN/94) with mo...

Xylanase J (XynJ) from alkaliphilic Bacillus sp. 41M-1 is an alkaline xylanase. The crystal structure has been solved with XynJ. Improvement of the alkaliphily of XynJ was attempted by amino acid substitutions. Reinforcing the characteristic salt bridge in the catalytic cleft and introducing excess Arg residues on the protein surface shifted the optimum pH of the wild-type enzyme from 8.5 to 9.5.

Acquired resistance to cisplatin has been generated in vitro in two human ovarian carcinoma cell lines: 41M, established from a previously untreated patient; and CHI, from a patient previously treated with cisplatin and cis-diammine-l,l-cyclobutane dicarboxylatoplatinum(II) (carboplatin). In neither cell line with acquired resistance did intracellular detoxification (via increased glutathione o...

Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.

Abstract. Geographical processes might well impact online engagement in big countries like the USA. Upon a random sample of 200K news articles and corresponding 41M comments posted on the Yahoo! News in that country, we show that nearby individuals tend to comment and engage with similar news articles more than distant individuals do. Interestingly, at state level, topics one reads about are as...

The mechanisms by which cis-diamminedichloroplatinum(II) (cisplatin) is transported across the plasma membrane (Le., passive diffusion versus active transport) were investigated in the 4!M and CH1 human ovarian carcinoma cell lines and their acquired cisplatin-resistant variants 4!McisR6 and CH!cisR6, respectively. Intracellular cisplatin accumuhation was significantly reduced (4.0 ± 1.7-fold) ...

We present ’empirical’ models (pressure vs. density) of Saturn’s interior constrained by the gravitational coefficients J2, J4, and J6 for different assumed rotation rates of the planet. The empirical pressure-density profile is interpreted in terms of a hydrogen and helium physical equation of state to deduce the hydrogen to helium ratio in Saturn and to constrain the depth dependence of heliu...