recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. end-product reutilization, low product concentration, large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2, 3-butanediol production. extraction can successfully be used f...

Recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. End-product reutilization, low product concentration, large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2, 3-butanediol production. Extraction can ...

The metabolism of millimolar concentrations of R- or S-1,3-butanediol has been studied in perfused livers from fed and starved rats. Protocols were designed to measure in the same experiment (i) uptake of the diol, (ii) the contribution of the diol to ketogenesis, (iii) the contribution of the diol to total fatty acid plus sterol synthesis, and (iv) conversion of S-1,3-butanediol into S-3-hydro...

Mutants of Alcaligenes eutrophus which are altered with respect to the utilization of 2,3-butanediol and acetoin were isolated after transposon mutagenesis. The suicide vehicle pSUP5011 was used to introduce the drug resistance transposable element Tn5 into A. eutrophus. Kanamycin-resistant transconjugants of the 2,3-butanediol-utilizing parent strains CF10141 and AS141 were screened for mutant...

The fermentation of different sugars by Bacillus stearothermophilus ATCC 2027 to obtain 2,3-butanediol is described. Fermentation tests in shake flasks show the complete conversion of sucrose to butanediol (91% yield) and acetoin (3-hydroxy-2-butanone) using a starting sugar concentration of 30 g/L. A similar result is obtained with sugar cane molasses, whereas glucose and fructose afford lower...

Bacillus licheniformis strain 3F-3 is an efficient pentose-utilizing producer of platform chemical, 2,3-butanediol. Here we present a 4.1-Mb assembly of its genome. The key genes for pentose utilization, regulation, and metabolism of 2,3-butanediol were annotated, which may provide further insights into the molecular mechanism of 2,3-butanediol production from biomass pentose.

Recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. End-product reutilization, low product concentration and large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2,3-butanediol production. Extraction can successfully be ...

Dividing wall column (DWC) is an advanced technology which could decrease energy consumption. As the vertical inserted in DWC increases degree of freedom systems, control becomes more difficult than conventional columns. In this study, mixture glycols (1, 2-propanediol+ 1, 3-butanediol+ 4-butanediol) were taken as feed, optimized structure was proposed, and three structures for tested by ± 10% ...

The completion of the Saccharomyces cerevisiae genome project in 1996 showed that almost 60% of the potential open reading frames of the genome had no experimentally determined function. Using a conserved sequence motif present in the zinc-containing medium-chain alcohol dehydrogenases, we found several potential alcohol dehydrogenase genes with no defined function. One of these, YAL060W, was o...

An actinomycete strain with a great potential to produce bioactive compounds isolated from a laterite soil was identified as Streptomyces sp. MSL based on 16S rDNA sequence analysis. Secondary metabolites produced by the strain in optimized nutrient broth were extracted and analyzed by chromatographic and spectroscopic techniques. Among the different fractions, four diols, viz., (1) (2R,3R)-2,3...