Monoclonal antibody (MAb) 2H1, raised in mice immunized with membrane fractions from cultured rat pheochromocytoma cells (clonal line PC-12), detects a polypeptide from rat brain and PC-12 cell membranes of 60-65 KD apparent molecular mass. The polypeptide has been localized by immunoelectron microscopy in the rough endoplasmic reticulum (RER) of neurons. By light microscopic immunocytochemistr...

We have recently identified peptide mimetics of the Cryptococcus neoformans capsular polysaccharide by screening phage display peptide libraries. 2H1, one of a large family of mAbs against the glucuronoxylomannan fraction (GXM), is highly protective and binds several peptide motifs. This study analyzes the immunologic properties of P601E (SYSWMYE), a peptide from the low affinity motif (W/YXWM/...

(R)- and (S)-4-methyl-[1-3H,2-2H1]pentanal were prepared from L- and D-leucine via leucic acid and (S)- and (R)-4-methyl-[2-2H1]pentanoic acid. Decarbonylation of these samples with tris-(triphenylphosphine)rhodium chloride followed by Kuhn-Roth oxidation of the resulting 2-methylbutane gave chiral acetic acid of 35% e.e. S and 31% e.e. R configuration, respectively. The decarbonylation reactio...

We address a question posed by Glatt-Holtz and Ziane in [GHZ09, Remark 2.1 (ii)], regarding moments of strong pathwise solutions to the Navier-Stokes equations in a two-dimensional bounded domain O. We prove that Eφ(‖u(t)‖2H1(O)) < ∞ for any deterministic t > 0, where φ(x) = log(1 + log(1 + x)). Such moment bounds may be used to study statistical properties of the long time behavior of the equa...

Cryptococcus neoformans causes a life-threatening meningoencephalitis in a significant percentage of AIDS patients. Mice immunized with a glycoconjugate vaccine composed of the glucuronoxylomannan (GXM) component of the cryptococcal capsular polysaccharide conjugated to tetanus toxoid (TT) produce Abs that, based on the epitope recognized, can be either protective or nonprotective. Since nonpro...

The interaction of Cryptococcus neoformans with the murine macrophage-like cell line J774.16 was studied in the presence and absence of monoclonal antibodies (MAbs) to the capsular polysaccharide glucuronoxylomannan (GXM). In the absence of MAb 2H1 to GXM, coincubation of J774.16 cells with C. neoformans reduced fungal colony-forming units in only 26.6% of 21 independent experiments. In the pre...

aspen: an experimental test of assisted migration Stefan G. Schreiber*, Chen Ding, Andreas Hamann, Uwe G. Hacke, Barb R. Thomas and Jean S. Brouard Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2H1, Canada; Alberta-Pacific Forest Industries, P.O. Box 8000, Boyle, AB, T0A 0M0, Canada; and Isabella Point Forestry Ltd., 331 Roland Road, Salt Spring Island, BC, V8K 1V1...