Abstract Background Valine and isoleucine are similar in chemical structure their limitation broiler chicken diets. To evaluate interactive effects, multivariate assessment nutrition studies for the branched-chain amino acids (BCAA) needed. A three level (− 1, 0, + 1), three-factor Box-Behnken design study was conducted to assess dietary BCAA ratios lysine of 65, 75, 85 valine, 58, 66, 74 isole...