Phytoplasmas associated with cucumber phyllody (CuP) and squash phyllody (SqP) in Yazd province of Iran were characterized by molecular analyses and biological studies. Orosius albicinctus leafhoppers testing positive for phytoplasma presence by polymerase chain reaction (PCR) successfully transmitted CuP and SqP phytoplasmas to healthy cucumber and squash plants. The phytoplasmas were also tra...

Phyllody, a destructive and economically important disease worldwide caused by phytoplasma infections, is characterized by the abnormal development of floral structures into stunted leafy parts and contributes to serious losses in crop plants, including sesame (Sesamum indicum L.). Accurate identification, differentiation, and quantification of phyllody-causing phytoplasmas are essential for ef...

Dracaena reflexa commonly known as “song of India” is an important ornamental plant grown both outdoors and a house plant. During October 2021, typical phytoplasma symptoms including little leaf stunting were observed in D. plants growing the landscape around Dr.Y.S.R. Horticultural University, Anantharajupeta, Andhra Pradesh. Eight diseased amongst 100 surveyed (Figure 1). Total DNA was extrac...

in 2010- 2012 surveys, witches'- boom disease of tomato was observed in borazjan area (bushehr province, iran). agent of the disease was transmitted from tomato to tomato and eggplant by grafting and to madagascar periwinkle via dodder inoculation, inducing phytoplasma-type symptoms in inoculated plants. presence of phytoplasma in naturally affected tomatoes and all symptomatic graft and dodder...

The bacterial genus "Candidatus Phytoplasma" contains a group of insect-transmitted plant pathogens in the class Mollicutes. Here, we report a draft genome assembly and annotation of strain NCHU2014, which belongs to the 16SrII-A subgroup within this genus and is associated with purple coneflower witches' broom disease in Taiwan.

Lime witches’ broom phytoplasma (16SrII, LWB) causes major losses to lime cultivation in Iran. The role of its immunodominant membrane protein (IMP) in the specific interaction with vector proteins was addressed. The carboxy terminal moiety of LWB IMP, coding the extracellular part of the protein, was expressed (LWB f∆IMP) as a tool to study IMP interactions with insect proteins. In preliminary...

Little leaf and yellowing symptoms associated with phytoplasma disease were observed in Pyracantha angustifolia Turkey. Amplification (through nested PCR) virtual restriction fragment length polymorphism (V-RFLP) confirmed the presence of symptomatic plants phylogeny based on R16F2n/R2 sequences P. was established sequence analysis showed that isolate had 98.7% similarity to ‘Candidatus Phytopl...