AIMS Changes in the expression of microRNAs (miRNAs) have been found in many cancers. This study aimed to investigate the expression of miR-1294 in patients with esophageal squamous cell carcinoma (ESCC) and its effect on prognosis. The underlying mechanism was explored as well. METHODS We examined the expression of miRNA in human ESCC cancer tissues and adjacent non-tumor controls using quan...

Desloratadine (Clarinex), the major active metabolite of loratadine (Claritin), is a nonsedating antihistamine used for the treatment of seasonal allergies and hives. Previously we reported that the formation of 3-hydroxydesloratadine, the major human metabolite of desloratadine, involves three sequential reactions, namely N-glucuronidation by UGT2B10 followed by 3-hydroxylation by CYP2C8 follo...

Replacement of hydrogenwith fluorine within three pairs of structurally similar small molecule inhibitors of heat shock protein 90 (HSP90) resulted in differences in inhibition constants (Ki) in vitro as well as marked differences in rat intravenous pharmacokinetic profiles. The difference in pharmacokinetic profiles between lower and higher affinity inhibitors (LAIs and HAIs, respectively) was...

Using in vitro drug sensitivity data coupled with Affymetrix microarray data, we developed gene expression signatures that predict sensitivity to individual chemotherapeutic drugs. Each signature was validated with response data from an independent set of cell line studies. We further show that many of these signatures can accurately predict clinical response in individuals treated with these d...

BACKGROUND Information was collected on 1500 obstetric fistula patients attending Bugando Medical Centre (BMC) in Mwanza, Tanzania. OBJECTIVES To identify high risk populations of fistula patients treated from 1998-2006. DESIGN A prospective description study of 1294 patients treated for urine and faecal incontinence at BMC. SETTING Obstetrics and Gynaecological ward at Bugando Medical Ce...

Simulation is important in evaluating novel methods when input data is not easily obtainable or specific assumptions are needed. We present , a cophesim software to add the phenotype to generated genotype data prepared with a genetic simulator. The output of can be used as a direct input for cophesim different genome wide association study tools. is available from cophesim . https://bitbucket.o...

Toxoplasma gondii is a unicellular parasite that causes severe brain and eye disease. Current drugs for T. gondii are limited by toxicity. Bumped kinase inhibitors (BKIs) selectively inhibit calcium-dependent protein kinases of the apicomplexan pathogens T. gondii, cryptosporidia, and plasmodia. A lead anti-Toxoplasma BKI, 1294, has been developed to be metabolically stable and orally bioavaila...

About 120 babies are born with cleft lip and palate in Finland each year. Most cases consist of isolated cleft palate (60%), while less than one in four cases suffer from complete cleft lip and palate. In Finland, clefts are closed before the child's first birthday. Clefts affect appearance, occlusion and speech, and the final outcome of treatment can only be assessed at the end of the child's ...

Despite the enormous economic importance of Neospora caninum related veterinary diseases, the number of effective therapeutic agents is relatively small. Development of new therapeutic strategies to combat the economic impact of neosporosis remains an important scientific endeavor. This study demonstrates molecular, structural and phenotypic evidence that N. caninum calcium-dependent protein ki...

Functional KATP (ATP-sensitive potassium) channels are hetero-octamers of four Kir6 (inwardly rectifying potassium) channel subunits and four SUR (sulphonylurea receptor) subunits. Possible interactions between the C-terminal domain of SUR2A and Kir6.2 were investigated by co-immunoprecipitation of rat SUR2A C-terminal fragments with full-length Kir6.2 and by analysis of cloned KATP channel fun...