The optimization of the Mapping Assay on the original Mapping 10K Array is described in the following Technical Note, along with additional experiments to cover development of the Mapping 10K 2.0 Array. In addition, concordance data between the original Mapping 10K Array and the Mapping 10K 2.0 Array is provided, allowing researchers to evaluate the performance of the Mapping Assay between the ...

A 3-stage pulse tube cooler has been designed to operate at 10¶K. The staged cooler uses the same compressor and parallel staging configuration for the 1 st and 2 nd stages as previously employed for our delivered High Capacity Cryocooler (HCC). The 2 nd and 3 rd stages are in a serial configuration. The addition of the third stage extends the performance of this cooler to lower operating tempe...

The 10K gene of simian herpes B virus (SHBV) has been located and the nucleotide sequence determined. Its relationship to homologous genes in other herpes-viruses has been examined. The SHBV 10K gene exhibits a closer relationship to its homologue in HSV-1 than to those in the other viruses studied. Nucleotide sequence identity of 61% was found between the HSV-1 and SHBV 10K genes, and 57% iden...

The Erd\H{o}s Pentagon problem asks to find an $n$-vertex triangle-free graph that is maximizing the number of $5$-cycles. was solved using flag algebras by Grzesik and independently Hatami, Hladk\'{y}, Kr\'{a}l', Norin, Razborov. Recently, Palmer suggested general $5$-cycles in $K_{k+1}$-free graphs. Using algebras, we show every order $n$ contains at most \[\frac{1}{10k^4}(k^4 - 5k^3 + 10k^2 ...

Resistance to cytotoxic nucleoside analogues is a major problem in cancer treatment. The cellular mechanisms involved in this phenomenon have been studied for several years, and some factors have been identified. Various strategies to overcome resistance have been suggested, but none has yet shown efficacy in vivo. We developed a gemcitabine-resistant cell line (L1210 10K) from the murine leuke...

The purpose of this study was to determine the relationship between selected anthropometric and physiological variables and 10K time. Eleven male wheelchair athletes with spinal cord injuries in training for national competition performed continuous progressive exercise tests on a wheelchair ergometer to determine maximal metabolic and cardiorespiratory values. Anthropometric data were also col...

The Genome 10K project aims to sequence the genomes of 10,000 vertebrates, representing approximately one genome for each vertebrate genus. Since fishes (cartilaginous fishes, ray-finned fishes and lobe-finned fishes) represent more than 50% of extant vertebrates, it is planned to target 4,000 fish genomes. At present, nearly 60 fish genomes are being sequenced at various public funded labs, an...