this study was conducted to evaluate the effect of egg yolk and cooling on ram coated spermatozoa. semen was collected from three ram by artificial vagina contacted with a tube containing tris- fructose-egg yolk 15%. samples were pooled, centrifuged by 700 g for 10 min and removed supernatant. then, samples were diluted by tris-glucose and centrifuged again to remove seminal plasma and egg yolk...

this experiment was conducted to evaluate the addition of seminal plasma to coated spermatozoa after 72 h. semen was collected from four taleshi rams by artificial vagina. to prepare coated spermatozoa, semen was collected in tube containing tris-fructose-15% egg yolk. after initial evaluation, the samples were mixed and centrifuged. after removal of supernatant and dilution, samples were divid...

in order to determine calcium (ca), magnesium (mg) content and total antioxidant capacity (tac) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its ca and mg content were estimated and its tac determined. the ca ...

background: as we all know, sperm has the capacity to take up foreign dna, therefore, sperm mediated gen transfer can be an inexpensive and simple method in animal transgenesis in various species. however, there is not sufficient evidence of dna uptake by ovine spermatozoa. objectives: the purpose of the present study was to examine the uptake of human lysozyme gene contained plasmid (pegfp-ire...

The ionic contents, pH and osmolality of seminal plasma and their relationships to sperm motility indices (sperm motility duration and percentage of motile sperm) were evaluated in bester. Semen was collected from six bester specimens and their seminal plasma was evaluated. The seminal plasma contained 54.5±2.8 mM sodium, 22.33±2.5 mM chloride, 7.1±1.2 mM potassium, 3.55±0.6 mM calcium and 0.58...

The ionic contents, pH and osmolality of seminal plasma and their relationships to sperm motility indices (sperm motility duration and percentage of motile sperm) were evaluated in bester. Semen was collected from six bester specimens and their seminal plasma was evaluated. The seminal plasma contained 54.5±2.8 mM sodium, 22.33±2.5 mM chloride, 7.1±1.2 mM potassium, 3.55±0.6 mM calcium and 0.58...

In order to determine calcium (Ca), magnesium (Mg) content and total antioxidant capacity (TAC) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its Ca and Mg content were estimated and its TAC determined. The Ca ...

in order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. the semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption spectrophotometer. the iron and lead content of the seminal plasma (mean ± sem) was recorded as...

Some biological aspects of semen were investigated in the Persian sturgeon, Acipenser persicus, by determination of seminal plasma indices (ionic composition and osmolality), sperm density and their relationships with sperm motility. The osmolality of seminal plasma ranged from 42.00 to 111.00 mOsmol Kg-1. The concentrations of Sodium (Na+), Potassium (K+), Chloride (Cl-), Calcium (Ca2+) and Ma...