The Ziehl-Neelsen stain is compared with three alternative methods of staining tubercle bacilli in paraffin sections: Fite's method (1938); a modification by Armstrong and Price (1947) of Fite's method; and a fluorescent method using the auramine-phenol stain. The fluorescent method was found to be the most effective of the four methods and its use is recommended as a routine tool in diagnostic...

mejoría. Acudió a consulta, con esta sintomatología, y debido al tamaño y la localización lateral de las úlceras, se le realizó biopsia de las mismas; el estudio histopatológico reportó la presencia de células gigantes tipo Langhans e intensa reacción granulomatosa (figs. 2 y 3), la prueba de PCR fue positiva para tuberculosis, se realizó tinción de Ziehl-Neelsen, la cual resultó positiva para ...

Materials and Methods: A total of 510 different extra pulmonary samples were collected during the period of August 2014 to July 2015. All the samples were subjected to Ziehl Neelsen staining. Samples were decontaminated by NALC-NaOH method and inoculated on a pair of Lowenstein-Jensen (LJ) media and one p-Nitrobenzoic acid (PNB). Cultures were incubated at 37 ̊C and observed for 8weeks for any g...

Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world ́s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study is to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of t...

This study describes aspects of the infection caused by the myxosporean genus Henneguya, which forms cysts in the bony portion of the gill filaments of Hypophthalmusmarginatus. Specimens of this catfish were acquired dead from artisanal fishermen near the town of Cametá, state of Pará, northern Brazil, between July 2011 and May 2012. They were transported in refrigerated containers to the Carlo...

Purpose. The histological diagnosis of Mycobacterium tuberculosis (MTB) remains a diagnostic challenge despite different methods. Immunohistochemistry (IHC) not only could confirm granulomatous tissue involvement but also can demonstrate MTB antigen immunolocalization. This study tries to clarify the details of immunohistochemical staining for MTB with pAbBCG. Materials/Methods. Twenty-three co...

The demand for tilapia fishing goods has recently expanded on a national scale. One of the difficulties this fish farming operation encountered was parasite-caused illness. aims study were to explore parasites in (Oreochromis niloticus) from local seller Dramaga, Bogor, West Java. A total 14 sampled, with an average length 22 cm and body weight 195 grams. Fish gills, pectoral fins, caudal tail ...