introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...

background: potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. potyviruses can be transmitted through plant sap, seeds and many aphid species. due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. objectives: this study compared the efficiencies of two couples of primers in thei...

The growth characteristics of Heterosigma akashiwo virus clone 01 (HaV01) were examined by performing a one-step growth experiment. The virus had a latent period of 30 to 33 h and a burst size of 7.7 x 10(2) lysis-causing units in an infected cell. Transmission electron microscopy showed that the virus particles formed on the peripheries of viroplasms, as observed in a natural H. akashiwo cell....

To efficiently generate varicella-zoster virus (VZV) mutants, we inserted a bacterial artificial chromosome (BAC) vector in the pOka genome. We showed that the recombinant VZV (VZV(BAC)) strain was produced efficiently from the BAC DNA and behaved indistinguishably from wild-type virus. Moreover, VZV's cell-associated nature makes characterizing VZV mutant growth kinetics difficult, especially ...

Background and Aims: Lemon Balm L (Lamiaceae) has been used in a variety of practical applications in medical sciences. Its antiviral activity against herpes simplex virus type-1 (HSV-1) was investigated in cell culture. Lemon Balm hydroalcoholic extract was found to be non-toxic to Vero cells up to concentration 800 ug/ml and inhibited the growth and development of HSV-1 in dose-dependent mann...

The rate of change of a microbial population is commonly depicted by a growth curve. This reflection of the dynamic aspects of reproduction under relatively fixed conditions is established readily for most microbial agents by interval enumeration either of the viable forms, or of the viable and nonviable forms. Contrariwise, for viruses, an accurate temporal enumeration of the intracellular and...

The growth of poliomyelitis virus, Type 2, Yale-SK strain, in cultures of monkey testicular tissue was observed to occur in discrete cycles. Growth curves showed that each cycle was composed of (a) an initial lag phase when little or no virus was released from the cells, (6) a phase of viral production, and (c) a plateau which represented a decrement in the rate of viral production. This patter...

Glycoprotein B (gB) plays an important role in alphaherpesvirus cellular entry and acts in concert with gD and the gH/gL complex. To evaluate whether functional differences exist between gB1 and gB4, the corresponding genes were exchanged between the two viruses. The gB4-containing-EHV-1 (EHV-1_gB4) recombinant virus was analyzed for growth in culture, cell tropism, and cell entry rivaling no s...

The rate of adsorption of WEE virus onto chicken embryo cells in vitro was determined both on a cell layer and on a cell suspension. One-step growth curves were determined in cell suspensions and on cell layers. The latent period varied between 2 and 3(1/2) hours; it was shorter on cell layers and decreased with higher multiplicity of infection. The shortest period is probably the real latent p...

The human cytomegalovirus UL82 gene encodes a protein (pp71) that is localized in the tegument domain of the virus particle. The UL82 gene product is delivered to the nucleus at the time of infection, and it is believed to function in gene activation. We have constructed a human cytomegalovirus mutant, ADsubUL82, that lacks a substantial portion of the UL82 coding region. It was propagated on h...