Metabolomics has become a mainstream analytical strategy for investigating metabolism. The quality of data derived from these studies is proportional to the consistency of the sample preparation. Although considerable research has been devoted to finding optimal extraction protocols, most of the established methods require extensive sample handling. Manual sample preparation can be highly effec...

The subject of metabolites in safety testing has had much debate in the recent past and has shown itself to be a complex issue with no simple solutions to providing absolute assurance of drug safety. Much of the attention has focused on the ability to identify metabolites and then demonstrate that their risk has been adequately characterized, either through their exposure in toxicology species ...

BACKGROUND The short-term infusion of levosimendan (Levo) improves hemodynamic function in patients with decompensated heart failure. The metabolites of Levo have a prolonged half-life, and one is hemodynamically active. The goal of this study was to determine whether the hemodynamic effects of Levo are sustained during a long-term infusion and beyond the discontinuation of drug infusion. MET...

Steroid profiling provides valuable information to detect doping with endogenous steroids. Apart from the traditionally monitored steroids, minor metabolites can play an important role to increase the specificity and efficiency of current detection methods. The applicability of several minor steroid metabolites was tested on administration studies with low doses of oral testosterone (T), T gel,...

Hypoglycaemia is a common, potentially fatal, yet preventable problem. The aetiology of hypoglycaemia is variable, but druginduced hypoglycaemia remains the commonest cause. Determining the aetiology of hypoglycaemia poses little difficulty in patients known to be taking oral hypoglycaemic agents (OHAs). However, it becomes a challenge in those without a history of such exposure. The Hospital A...

Children with mental retardation are often subjected to a routine battery of tests, a practice that has been criticised.' Investigations often include a blood count, a biochemical profile, urinary screening for metabolites including amino acids, and radiography of the skull and wrist for bone age and may be more extensive. Such routine testing of all children with mental retardation is done in ...

An immunoaffinity chromatographic (IAC) method for isolating sulfamethazine (SMZ) from incurred urine samples was developed. This was achieved by (i) generating polyclonal antibodies that recognize equally well SMZ and its major urinary metabolites, (ii) evaluating in an ELISA procedure the influence of methanol, salt and pH on the antigen-antibody interaction in order to determine the optimum ...

OBJECTIVE: This study was carried out to determine the serum metabolite antioxidants concentration(Vitamins C and E and other cofactors such as zinc and iron) serving as indicesand correlated with the anti-microsomal antibody level, which is one of the markers and causes of infertility

objective(s):  medicinal benefits of quantum dots have been proved in recent years but there is little known about their toxicity especially in vivo toxicity. in order to use quantum dots in medical applications, studies ontheir in vivo toxicity is important.  materials and methods:cdse:zns quantum dots were injected in 10, 20, and 40 mg/kg doses to male mice10 days later, mice were sacrificed ...