The transmission disequilibrium test (TDT) is a useful method to locate mutations linked to disease genes associated with complex diseases. TDT requires genotypes of affected individuals and their parents. Recently, Ewens and Spielman (Am J Hum Genet 1998;62:450-8) extended the TDT for use in sibships with at least one affected and one unaffected individual and devised a new test called the sib...

Acute mixed myeloid-lymphoid leukemia is uncommon. We report four cases in which myeloid and lymphoid cell markers were observed simultaneously or sequentially when 94 patients with acute leukemia were phenotyped according to the French-American-British (FAB) classification system, with cytochemical stains, and with immunologically defined differentiation markers (identified by monoclonal antib...

OBJECTIVE To present an alternative linkage test to the transmission/disequilibrium test (TDT) which is conservative under the null hypothesis and generally more powerful under alternatives. METHODS The exact distribution of the TDT is examined under both the null hypothesis and relevant alternatives. The TDT is rewritten in an alternate form based on the contributions from each of the three ...

In linkage analysis for mapping genetic diseases, the transmission/disequilibrium test TDT uses the linkage disequilibrium LD between some marker and trait loci for precise genetic mapping while avoiding confounding due to population stratification. The sib-TDT S-TDT and combinedTDT C-TDT proposed by Spielman and Ewens can combine data from families with and without parental marker genotypes PM...

We present the design and development of a Hidden Markov Model for the division of news broadcasts into story segments. Model topology, and the textual features used, are discussed, together with the non-parametric estimation techniques that were employed for obtaining estimates for both transition and observation probabilities. Visualization methods developed for the analysis of system perform...

Abstract Topic Detection and Tracking (TDT) is a DARPA-sponsored initiative concerned with finding groups of stories on the same topic (tdt, 1998). The goal is to build systems that can segment, detect, and track incoming news stories (possibly from multiple continuous feeds) with respect to pre-defined topics. While the detection task detects the first story on a particular topic, the tracking...

Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase that does not require a template, is found in thymocytes and marrow Tlymphocytes normally [1,2]. Shortly after these discoveries, McCaffrey et al. [3] described the presence of this enzyme in the leukemic cells of patients with acute lymphoblastic leukemia (ALL) and Sarin et al. [4] discovered that some patients with chronic m...

To understand the mechanisms controlling vein patterning in Arabidopsis thaliana, we analyzed two phenotypically similar mutants, varicose (vcs) and trident (tdt). We had previously identified VCS, and recently, human VCS was shown to function in mRNA decapping. Here, we report that TDT encodes the mRNA-decapping enzyme. VCS and TDT function together in small cytoplasmic foci that appear to be ...

Bone marrow cells were examined by double immunofluorescent labeling techniques to detect determinants for the B lineage monoclonal antibody, 14.8, the nuclear enzyme, terminal deoxynucleotidyl transferase (TdT), cytoplasmic mu chains (c mu), and surface mu (s mu). In 8-9-wk-old C3H/HeJ mice, 14.8+ cells totalled 22.2% of all marrow cells (35 X 10(5) cells/femur). While many 14.8+ cells were c ...

MicroRNAs (miRNAs) constitute a critically important class of non-translated, small RNAs, which post-transcriptionally regulate gene expression via one of the multiple mechanisms. To profile miRNA expression, microarrays have been extensively applied to the high-throughput detection of miRNAs. Here, we described a novel 3'-end miRNA-labeling method for microarray detection by terminal-deoxynucl...