This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The is designed to enhance the stability of metastable supercooling state, offering potential long-term large biological organs at subfreezing temperatures without need for cryoprotectant additives. Detailed designs and usage protocols are provided researchers interested in explo...

Flat grain beetles (Coleoptera: Laemophloeidae) are common stored-product insect pests in Canada, infesting cereals bins, equipment and end products flour mills. We studied the cold tolerance of three most flat beetles: Cryptolestes ferrugineus, turcicus pusillus, by measuring survival at −10 °C supercooling point (SCP) for different life stages (egg, young larva, old pupa adult) reared on mixe...

The freezing behaviour studies of dormant buds, were examined, employing scanning electron microscopy (SEM) and light microscopy. The differences and effect of freezing behaviour on dormant buds were observed. The dormant bud primordia of several woody plant species avoid freezing injury by deep supercooling. By slow cooling (5C/day) of dormant buds to –30C, all living cells in bud tissues exhi...

Hatchling painted turtles (Chrysemys picta) commonly hibernate in shallow, natal nests where winter temperatures may fall below -10 degrees C. Although hatchlings are moderately freeze-tolerant, they apparently rely on supercooling to survive exposure to severe cold. We investigated seasonal changes in physiology and in the development of supercooling capacity and resistance to inoculative free...

The European common lizard (Lacerta vivipara) tolerates long periods at sub-zero temperatures, either in the supercooled or the frozen state. Both physiological conditions limit oxygen availability to tissues, compelling lizards to cope with potential oxidative stress during the transition from ischemic/anoxic conditions to reperfusion with aerated blood during recovery. To determine whether an...

Current theory indicates that the structure of the cell wall is integral to the ability of a tissue to exhibit deep supercooling. Our previous work has indicated that the structure of the pit membrane and/or amorphous layer (protective layer), rather than the entire cell wall, may play a major role in deep supercooling (21, 22). The present study indicates a shift in the low-temperature-exother...

It has been accepted that xylem ray parenchyma cells (XRPCs) in hardwood species respond to subfreezing temperatures either by deep supercooling or by extracellular freezing. Present study by cryo-scanning electron microscopy examined the freezing responses of XRPCs in five boreal hardwoods: Salix sachalinensis Fr. Schmit, Populus sieboldii Miq., Betula platyphylla Sukat. var japonica Hara, Bet...