BACKGROUND Sulphites are widely used as a preservative and antioxidant additives in the food and pharmaceutical industries. Many types of biological and toxicological effects of sulphites in multiple organs of mammals have been shown in previous studies. OBJECTIVE The aim of this study was to investigate the effects of sodium metabisulfite (SMB) on testicular function and morphometric values ...

During the past 15 years, digoxin has been routinely measured in serum by various immunoassays. Unfortunately, all of the currently available immunoassays exhibit various cross reactivities with drugs, digoxin metabolites, and endogenous digoxin-like immunoreactive substances (1, 2). We recently encountered several patients for whom we were unable to obtain a reportable digoxin concentration wi...

E NEED for an accurate and simple determination for serum glutamic oxalacetic transaminase (SGO-T) in the clinical laboratory led! us to investigate a procedure published recelltiy by Babson et al. (1,2). III this niethod the (hiazonium salt, (i-bellzamido-4-methoxv-ln-tolui(line (liazoluunl chloride, pro(111c’s a higilly specific color for the oxalacetic acid! fornied as a product of transamin...

Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity ...

The effect of formulation factors on permeation of diclofenac from some experimental and marketed aqueous eye drops through excised goat cornea was evaluated. Raising the pH of formulation from 6.0 to 8.0 or diclofenac concentration from 0.05 to 0.15% (w/v) or adjusting tonicity with mannitol or addition of viscolizing agent decreased apparent permeability coefficient (Papp). Formulation (pH 7....

Thermal processing of green coconut water (GCW) caused non-enzymic browning and development of rancidity. Effect of the addition of several combinations of ascorbic acid (AA) (0 to 100 ppm) and sodium metabisulfite (SMB) (0 to 30 ppm) on brown discolouration and rancidity of GCW during elevated thermal processing (121°C for 5 min at 15 psi) was investigated. Addition of AA and/or SMB significan...

The effectiveness of pineapple juice in enzymatic browning inhibition was evaluated on the cut surface of banana slices. After storage of banana slices at 15 degrees C for 3 days, pineapple juice showed browning inhibition to a similar extent as 8 mM ascorbic acid but less than 4 mM sodium metabisulfite. Fractionation of pineapple juice by a solid-phase C18 cartridge revealed that the directly ...