Mesophyll protoplasts and bundle sheath strands of maize (Zea mays L.) leaves have been isolated by enzymatic digestion with cellulase. Mesophyll protoplasts, enzymatically released from maize leaf segments, were further purified by use of a polyethylene glycol-dextran liquid-liquid two phase system. Bundle sheath strands released from the leaf segments were isolated using filtration techniques...

AbsractA rare variant of ansa cervicalis was discovered during dissection of the neck in amale cadaver. The superior root of the Ansa cervicalis was formed by the C1 ventralramus. It accompanied with the vagus nerve in place of the hypoglossal nerve and descendedinto the carotid sheath with it. Moreover the C1 ventral ramus formed fromthe sheath and joined the inferior root from C2 and C3 ventr...

multiple sclerosis (ms) causes loss of the myelin sheath, which leads to loss of neurons. regeneration of myelin sheath stimulates axon regeneration and neurons’ survival. in this study, olfactory ensheathing cell (oec) transplantation is investigated to restore myelin sheath in an experimental model of ms in male mice.oecs were isolated from the olfactory mucosa of seven-day-old infant rats an...

Both extrinsic and intrinsic tissues contribute to tendon repair, but the origin and molecular functions of extrinsic tissues in tendon repair are not fully understood. Here we show that tendon sheath cells harbor stem/progenitor cell properties and contribute to tendon repair by activating Hedgehog signaling. We found that Osteocalcin (Bglap) can be used as an adult tendon-sheath-specific mark...

In order to analyze electron sheath formation we performed two dimensional PIC (Particle In Cell) simulations in magnetized plasmas. We investigated the influence of the probe geometry on the electron sheath formation in terms of the aspect ratio of the plane probe. The dependence of the magnetic field and voltage bias of the probe on the electron sheath were also taken into consideration. The ...

The bacterial Type VI secretion system (T6SS) delivers proteins into target cells using fast contraction of a long sheath anchored to the cell envelope and wrapped around an inner Hcp tube associated with the secreted proteins. Mechanisms of sheath assembly and length regulation are unclear. Here we study these processes using spheroplasts formed from ampicillin-treated Vibrio cholerae. We show...

Sphaerotilus natans is a filamentous sheath-forming bacterium. A method of selective fluorescent-labeling of its sheath using conventional reagents was developed. Terminal expansion of the sheath was confirmed by this method. In addition, ubiquitous cell growth was revealed by sequential phase-contrast microscopy of a filament. Based on this and earlier reports, a model of the sheath formation ...

Optic nerve sheath meningiomas and optic nerve gliomas can be difficult to differentiate by computed tomography (CT). Three patients with optic nerve sheath meningiomas were studied with a CT/T 8800 scanner with 1.5-mm-thick axial sections and intravenous contrast material. In each case, an abnormal optic nerve sheath was identified by CT. A soft-tissue mass arose from one side of the sheath in...

An experiment was conducted at Institute of Agriculture and Animal Science (IAAS), Rampur, Nepal during July 2011 to September 2011 with an objectine to determine phenotypic variability of Nepalese finger millet landraces using descriptive statistics, cluster analysis and principal component analysis. FValue of REML procedure of mixed model analysis revealed that highly significant variation wa...

Sheath blight (ShB) caused by Rhizoctonia solani is one of the serious constraints that hamper global rice production. It causes severe yield loss up to 69% under favorable environmental conditions. The application chemical fungicides remains primary approach for management this disease. But, overuse potential health and risk. use host plant resistance a very effective, economic, sustainable st...